绿色荧光蛋白(ZsGreen)基因在长石莼细胞中的表达  

Expression of Green Fluorescent Protein(ZsGreen)in Ulva linza

作　　者：何建华[1,2] 汤文仲[2] 叶静[2] 田琪琳[2,3] 何培民[2,3] 

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]上海海洋大学水产与生命学院,上海201306 [3]上海海洋大学海洋科学研究院,上海201306

出　　处：《生物技术通报》2011年第9期114-119,共6页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(30371101);国家"863"计划资助项目(2009AA064401)

摘　　要：主要研究绿色荧光蛋白(ZsGreen)基因在长石莼(缘管浒苔)细胞中的表达。应用绿色荧光蛋白基因、抗除草剂bar基因、CMV 35S启动子和SV40双启动子构建了质粒载体PSV-bar-ZsGeen,采用改进的PEG法将质粒载体PSV-bar-Zs-Geen导入到缘管浒苔原生质体中,经过细胞培养发育再生藻株,通过除草剂筛选出阳性藻株,且转化率达38.58%,进一步PCR分子检测和显微荧光检测表明,绿色荧光蛋白基因在转基因植株中得到表达,为今后转基因浒苔研究奠定基础。In this paper,expression of green fluorescent protein（ZsGreen）in Ulva linza was studied.The PSV-bar-ZsGeen expression vector was successfully constructed,which contained PPT resistance gene bar,the promoter of CMV 35S and SV40.With improved PEG method,the PSV-bar-ZsGreen vectors were transferred into protoplasts of Ulva linza and the transferred protoplasts were cultured and regenerated into younger plants.The transferred cells（or plants）resisting to Basta were selected,and the relative transformation rate reached up to 38.58%.The further study successfully constructed the detection system with PCR technology and Fluorescence microscope OLYMPAS IX71 on the lever of gene and protein.All these studies would built foundation for the further research on transgenic Ulva linza.

关 键 词：GFP PEG转化 缘管浒苔 报告基因 

分 类 号：Q943.2[生物学—植物学]