miR-125b、miR-331和miR-365在疼痛中的表达变化及其miRNA靶标预测  

Expression of miRNAs and prediction of their targets in diffferent pain models

作　　者：潘志强[1] 朱丽娇[1] 杨俊霞[1] 郝凌云[1] 曹君利[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《中国医药导报》2011年第27期18-20,24,共4页China Medical Herald

基　　金：国家自然科学基金(项目编号:81070888);东南大学生物电子学国家重点实验室开放课题;徐州医学院院长专项人才基金(项目编号:09KJZ06)

摘　　要：目的:在慢性疼痛、神经病理痛和急性痛3种模型中定量检测和分析神经特异性miR-125b、miR-331和miR-365的表达,并结合生物信息学预测表达差异较大的miRNA的靶标基因。方法:从疼痛模型SD大鼠的脊髓和背根中分别提取miRNA,用实时定量PCR(RT-qPCR)方法对其进行定量分析。用生物信息学软件分析靶标基因。结果:miR-125b、miR-331和miR-365在不同的疼痛模型中脊髓和背根中均有表达,但表达差异并不一致,其中miR-125b表达差异最显著。miR-125b在慢性炎性痛、神经病理痛和急性炎性痛动物的脊髓中分别被下调0.9倍、上调0.89倍和下调2.57倍;在背根中分别被下调1.78倍、上调0.54倍和上调0.47倍。信息学分析表明miR-125b作用的可能靶标基因为核孔蛋白210(NUP210)多梳基环指蛋白6(Pcgf6),肿瘤坏死因子配体超家族成员4(Tnfsf4),Bcl2蛋白修饰因子(BMF),可溶性载质转运蛋白35成员4(Slc35a4)和液泡蛋白质分选因子4B(VPS4B),它们可能与疼痛发生和维持相关。结论:miR-125b可能与疼痛发生和维持相关。Objective： To analyze the expression of miR-125b,of miR-331 and of miR-365 in three different SD rat pain models including CFA pain model,neuro-pathology pain and acute pain model,respectively;and predict the potential targets of the miRNAs with most difference.Methods： RT-qPCR was used to detect and analyze the expression of these miRNAs.Three bioformatics softwares including Target,PicTar and RNA22 software were ultilzed to predict the miRNA targets.Results： Three miRNAs were expressed in the spinal cord（SC） and doral root ganglion（DRG） of three different pain models.The difference of miR-125b expression were comparatively obvious in SC and DRG of three pain models.The analysis of bioinformatics indicated the targets NUP210,Pcgf6,Tnfsf4,BMF,Slc35a4 and VPS4B for miR-125b were impossibly closely associated with the methanism of development and maintance of pain.Conclusion： Pcgf6,Tnfsf4,BMF,Slc35a4 and VPS4B for miR-125b may paticipate in the machanism of pain development and maintance.

关 键 词：疼痛 MIRNA 靶标 

分 类 号：R411.1[医药卫生—临床医学]