高原肺水肿动物模型的初步建立  

The primary estibalishment of rats model of high-altitude pulmonary edema

作　　者：张兆瑞[1] 张波[1] 何萍萍[1] 王东[1] 

机构地区：[1]空军总医院呼吸内科,北京100142

出　　处：《中国医药导报》2011年第27期27-28,66,共3页China Medical Herald

摘　　要：目的:通过模拟高原低压低氧环境,建立高原肺水肿大鼠动物模型,探讨低氧时间对大鼠肺水肿程度影响。方法:雄性Wistar大鼠随机分为3组,分别为对照组、24 h低氧组、48 h低氧组。比较大鼠肺湿干比、肺组织匀浆中一氧化氮合酶(NOS)、内皮素-1(ET-1)及肺组织病理形态学的变化。结果:与地面对照组比较,24 h低氧组与48 h低氧组大鼠肺湿干比及肺组织匀浆内ET-1升高、NOS降低,差异均有统计学意义(均P<0.05);病理学显示,24 h低氧组与48 h低氧组均有肺泡间隔增宽、间质充血等间质性肺水肿表现,与24 h低氧组相比,48 h低氧组肺湿干比、ET-1升高,肺组织匀浆内NOS降低,差异有统计学意义(P<0.05)。结论:低压低氧可以有效诱导大鼠产生间质性肺水肿,且48 h低氧大鼠肺水肿程度较24 h低氧更严重,更适合应于现实研究。Objective： To estibalish the rats model of high-altitude pulmonary edema by putting Wistar rats in hypobaric chamber,and compare the severity of pulmonary edema in different hypoxia time.Methods： Male Wistar rats were randomly divided into three groups： control group,24 h hypoxia group,48 h hypoxia group.Except for the control group,rats in the other groups were put in hypobaric chamber for 24 h and 48 h to produce a rat model of high-altitude pulmonary edema（HAPE）.The wet to dry ratio（W/D）,lung oxide synthase（NOS）,lung endothelin（ET-1） and histological measurements were conducted on each animal.Results： Compared with the control group,the W/D ratio and the ET-1 had significantly increased,the activity of NOS was lower significantly in the 24 h hypoxia group and 48 h hypoxia group,and they all had statistically significant differences（P〈0.05）.The interstitial pulmonary edema performance of the alvedar interval widened and interstitial hyperemia were found in the 24 h and 48 h hypoxia group.Comparied with the 24 h hypoxia group,the wet to dry ratio and the endothelin had significantly increased,the activity of NOS were significantly lower of the 48 h hypoxia group,and there was a statistically significant difference（P〈0.05）.Conclusion： The model of high altitude pulmonary interstitial edema is successful set up by putting the Wistar rats in hypobaric chamber for 24 h and 48 h,the severity of pulmonary edema in the 48 h hypoxia group is more serious than the 24 h hypoxia group,which is more suitable for reality research.

关 键 词：高原病 肺水肿 大鼠 一氧化氮合酶 内皮素-1 

分 类 号：R332[医药卫生—人体生理学]