多溴联苯醚对鲫鱼组织DNA损伤及p53蛋白表达的影响  

Effects of Polybrominated Diphenyl Ethers on DNA Damage and p53 Protein Expression in Carassius auratus Linn

作　　者：吴伟[1,2] 瞿建宏[1] 陈家长[1,2] 聂凤琴[2] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心,中国水产科学研究院内陆渔业生态环境与资源重点开放实验室,江苏无锡214081 [2]南京农业大学渔业学院,江苏无锡214081

出　　处：《农业环境科学学报》2011年第9期1836-1841,共6页Journal of Agro-Environment Science

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2007JBFB02);中国水产科学研究院内陆渔业生态环境与资源重点开放实验室开放课题(YM2007-10)

摘　　要：以鲫鱼(Carassius auratus)为试验材料,研究了经不同浓度的2,2’,4,4’-四溴联苯醚(PBDE-47)和十溴联苯醚(PBDE-209)暴露后,鲫鱼体内DNA损伤产物8-羟基脱氧鸟苷(8-OHdG)的含量及p53蛋白的水平。结果表明,采用浓度为0.10～5.00mg·L-1的PBDE-47和10.0～50.0mg·L-1的PBDE-209处理鲫鱼20d,除了0.10mg·L-1PBDE-47和10.0mg·L-1PBDE-209浓度组的8-OHdG和p53蛋白含量与对照组相比无显著差异(P>0.05),其余各浓度组的8-OHdG和p53蛋白含量均随PBDE-47和PBDE-209浓度增加而逐渐上升,呈显著的相关关系(P<0.01),且8-OHdG和p53蛋白之间也呈显著的正相关。说明PBDE-47和PBDE-209对鲫鱼组织的DNA产生了损伤,具有遗传毒性影响。Persistent organic pollutants（POPs） has a long-term threat to different biota, including aquatic animals, mammals, especially humans, due to their biomagnified effect in food chain, the study on the toxic effect of POPs to aquatic animals was evidently important and concerned. This paper discussed the variation of DNA-damage product 8-Hydroxy-desoxyguanosine（8-OHdG） and p53 protein to Carassius auratus in exposed to poly -brominated diphenyl ethers （PBDE-47 and PBDE-209 ）, where the exposed dose of PBDE-47 and PBDE- 209 ranged from 0.10 to 5.00 mg· L^-1 and 10.0 to 50.0 mg· L^-1, respectively. The results showed that, DNA-damage product 8-OHdG and p53 protein were generated extraordinary in all treatment lasted for 20 days, expect the 0.1 mg· L^-1in PBDE-47 and 10.0mg· L^-1 in PBDE-209. The variation of 8-OHdG and p53 protein to Carassius auratus when exposed to PBDE-47 and PBDE-209 were significantly dose-depend （P〈0.01）, respectively, and the variation of 8-OHdG was significantly correlated with the variation of p53 protein as well. The results indicated that, the genotoxic effect, characterized by DNA-damage product, such as 8-OHdG and p53 protein in Carassius auratus when exposed to PBDE-47 and PBDE-209 were produced. The paper provided more scientific details to the research on the toxic effect, especially genotoxic effect of POPs to aquatic animals, with a result to recognize, control and eliminate POPs in different environmental medium.

关 键 词：多溴联苯醚 鲫鱼 DNA 损伤 P53蛋白 

分 类 号：X503.225[环境科学与工程—环境工程]