温补肾阳方对创伤后应激障碍大鼠行为学及血浆17-OHCS、cAMP影响的研究  

Study on the Effect of Prescription for Warming and Recuperating the Kidney-Yang on the Ethology and 17-OHCS,cAMP in Plasma of PTSD Rat

作　　者：张鹏[1] 谢磊[1] 罗瑞[1] 江泳[1] 

机构地区：[1]成都中医药大学基础医学院,四川成都611137

出　　处：《四川中医》2011年第9期19-21,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：成都中医药大学2009年校基金自然科学一般项目(编号:ZRYB200925)

摘　　要：目的:探讨温补肾阳方对创伤后应激障碍(posttraumatic stress disorder,PTSD)大鼠行为学及血浆17-OHCS、cAMP的影响。方法:选择体重和旷野试验(open-field test)评分相近的36只SD大鼠,随机分为空白组、PTSD模型组、温补肾阳组,应用分养和国际上已确认可作为制备PTSD动物模型的SPS法造大鼠PTSD模型,定期测定各组大鼠行为学,采用酶联接免疫吸附剂(ELISA)法检测血浆17-OHCS、cAMP含量,观察温补肾阳方对该模型大鼠的疗效。结果:PTSD模型组大鼠Open-Field评分、糖水摄取量、17-OHCS、cAMP明显低于空白组(P<0.05或P<0.01);治疗组可改善PTSD大鼠Open-Field评分、糖水摄取量、17-OHCS、cAMP,与模型组相比,有统计学差异(P<0.05)。结论:温补肾阳方对创伤后应激障碍的老鼠有改善作用。Objective：To Study on the effect of prescription for warming and recuperating the kidney-Yang on the ethology and 17-OHCS、cAMP in plasma of PTSD rat.Methods：36 adult SD rats which were similar in weight and open-field test grade were randomly divided into a normal group,a PTSD model group and the WBSYF group.Separated feeding,SPS method which was verified by internation were used for developing PTSD rat model.Measuring rats＇behavior at regular intervals and 17-OHCS,cAMP in plasma through ELISA method.To observe the effect on PTSD rat model treated by prescription for warming and recuperating the kidney-Yang.Results：The score of Open-Field,sucrose solution consumption,17-OHCS and cAMP of PTSD model group was obviously lower than that of normal group（P0.05 or P0.01）;the scores of Open-Field,sucrose solution consumption,17-OHCS and cAMP of treatment groups improved much better than PTSD model group,There was significant difference between PTSD model group and treatment group（P0.05）.Conclusion：The findings have indicated that the efficacy of prescription for warming and recuperating the kidney-Yang has positive effect of PTSD rats.

关 键 词：温补肾阳方 PTSD 行为学 17-OHCS CAMP 

分 类 号：R285.5[医药卫生—中药学]