文雅杜鹃组织培养研究  

Study on Tissue Culture of Rhododendron facetumBalf.f.et Ward

作　　者：陈平芬 刘家迅[2] 李兴贵[3] 高飞[2] 梁明泰[2] 

机构地区：[1]昆明金科艺花卉公司,云南昆明650213 [2]云南省农业科学院园艺研究所,云南昆明650205 [3]中国科学院昆明植物研究所,云南昆明650204

出　　处：《北方园艺》2011年第17期134-136,共3页Northern Horticulture

基　　金：国家星火计划资助项目(2010GA830033);昆明市科技计划资助项目(10S040204)

摘　　要：以文雅杜鹃1a生半木质化茎段作外植体,以WPM为基本培养基,研究不同激素浓度和组合对文雅杜鹃不定芽诱导和生根的影响。结果表明:外植体在WPM+ZT 2.0mg/L(单位下略)+IAA 0.5添加蔗糖30g/L,琼脂4g/L,pH 5.0的培养基上诱导率达83.33%;最佳继代培养基为WPM+ZT 0.8+NAA 0.05+蔗糖30g/L+琼脂4g/L,pH 5.0;最适生根培养基为WPM+IBA 1.0+NAA 0.5+蔗糖30g/L+琼脂7g/L+活性炭3g/L,pH 5.0,生根率为93%。Taking the semi-lignification stems segments of annual Rhododendron facetum Balf.f.et Ward as explants and WPM as the basic medium to study on the effect of different hormone concentrations and combinations on bud induction and rooting of Rhododendron facetum Balf.f.et Ward.The results showed that the best medium for explants induction was WPM＋ZT 2.0＋IAA 0.5 add sucrose 30 g/L,agar 4 g/L,pH 5.0.The stem segments of Rhododendron facetum Balf.f.et Ward on inductivity had highly reached 83%.The best multiplication medium was WMP＋ZT 0.8 mg/L＋NAA 0.05 mg/L add sucrose 30 g/L,agar 4 g/L,pH 5.0.The optimal rooting medium was WMP＋ IBA 1.0 mg/L＋ NAA 0.5 mg/L,add sucrose 30 g/L,agar 7 g/L＋ activated carbon 3 g/L,pH 5.0,rooting rate was 93%.

关 键 词：文雅杜鹃 组织培养 繁殖 

分 类 号：S685.21[农业科学—观赏园艺]