沂州木瓜初代培养技术研究  

Studies on the Initial Culture Technology of Yizhou Pawpaw

作　　者：杨凤玲[1] 

机构地区：[1]临沂大学实验中心,山东临沂276000

出　　处：《北方园艺》2011年第17期144-145,共2页Northern Horticulture

摘　　要：利用沂州木瓜新长出10d左右的嫩茎为外植体,采用不同消毒剂、灭菌时间、不同浓度6-BA对其进行初代培养试验。结果表明:沂州木瓜最佳消毒药剂及时间是70%酒精10s+0.1%升汞2～4min。当NAA浓度为0.05mg/L时,沂州木瓜试管苗生长适宜的6-BA浓度为0.5mg/L。The 10 days new buds of Yizhou pawpaw were used as the explants.These explants were cultured primarily with different disinfectors and different sterilizing time and different concentration of 6-BA.The results showed that it was the best for the explants to treat with 70% alcohol for 10 s and 0.1% HgCl2 for 2~4 min.When the concentration of NAA was 0.05 mg/L,the suitable concentration of 6-BA for Yizhou pawpaw test-tube plantlet growth was 0.5 mg/L.

关 键 词：沂州木瓜 细胞分裂素 丛生芽 外植体 

分 类 号：S661.6[农业科学—果树学]