Nitric Oxide Production is Involved in Inhibition Growth of Salicylate at Chilling Temperature  

低温条件下一氧化氮参与了水杨酸对拟南芥生长的抑制作用(英文)

作　　者：夏金婵[1,2] 何奕昆[2] 

机构地区：[1]河南中医学院,河南郑州450008 [2]首都师范大学,北京100048

出　　处：《Agricultural Science & Technology》2011年第9期1283-1285,共3页农业科学与技术（英文版）

基　　金：Supported by National High Technology Research and Development Program of China(863Program,No.2002AA2Z1001);National Natural Science Foundation of China(30328003);Doctoral Scientific Research Fund of Henan University of TCM~~

摘　　要：[Objective] The aim was to investigate whether NO is involved in the inhibitory effect of salicylic acid on the growth at low temperature in Arabidopsis.[Method] The endogenous NO level in roots of wild-type and NahG mutant growing at 22 or 4 ℃ was measured with NO-specific probe DAF-FM DA staining method.[Result] NO level in plants growing at 4 ℃ for a long term could be increased with time;low temperature could induce NO production in wild-type and NahG mutant,and the NO level in NahG mutant with low level of salicylic acid was significantly lower than that in wild-type.[Conclusion] NO was involved in the inhibitory effect of salicylic acid on the growth of Arabidopsis at low temperature.[目的]验证NO是否参与了水杨酸对拟南芥生长的抑制过程,为植物在长期低温下生长的分子机制研究奠定基础。[方法]用可渗透细胞膜的NO特异性探针DAF-FMDA染色法测定4℃低温处理下,野生型拟南芥(Arabidopsis thaliana)和NahG突变体根中内源NO含量的变化。[结果]长期低温4℃处理可诱导植物中NO含量增加,并随时间的延长而升高;低温能够诱导野生型和NahG突变体中NO的产生,但体内水杨酸含量低的NahG突变体中NO含量明显低于比野生型。[结论]低温条件下NO参与了水杨酸对拟南芥生长的抑制过程。

关 键 词：Arabidopsis thaliana Low temperature Salicylic acid Nitric oxide 

分 类 号：Q946[生物学—植物学]