Effect of Different Size of Ground Cyanotis arachnoidea Particles on the Release of β-ecdysone  

露水草加工粒径和其β-蜕皮激素释放的关系(英文)

作　　者：朱国栋[1] 张易祥[1] 叶金云[1] 吴成龙[1] 于岚[1] 杨霞[1] 

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000

出　　处：《Agricultural Science & Technology》2011年第9期1318-1319,1326,共3页农业科学与技术（英文版）

基　　金：Supported by Major Project for International Cooperation in Zhejiang(2009C14019);"948"Project of Ministry of Agriculture(2010-Z40);Major Project from Department of Education of Zhejiang(ZD2009009);Huzhou Major Science and Technology Project(2010ZD1002);the National Natural Science Foundation of China(20977027);Science and Technology Innovation Program for Under graduate Students in Zhejiang Province(2011R425009)~~

摘　　要：[Objective] The aim was to investigate the influence of different size of ground Cyanotis arachnoidea C.B.Clarke particles on the release of β-ecdysone.[Method] The content of β-ecdysone extracted from Cyanotis arachnoidea powders of different sizes was detected through HPLC,under the chromatographic conditions：GRACE ODS C18 column（250 mm × 4.6 mm,5 μm）,mobile phase methanol：water= 40：60（V/V）,column temperature 26 ℃,flow rate 1.0 ml/min and detection wavelength 248 nm.[Result] The extracted β-ecdysone from ultra-fine Cyanotis arachnoidea powder（particle size 10,30 and 50 μm） was as more than two folds as common powder（particle size 180 μm）.[Conclusion] The β-ecdysone in ultra-fine powder of Cyanotis arachnoidea was more easily soluble in methanol.The extraction rate of β-ecdysone was significantly higher than that of common powder.The result showed that the technology of ultra-fine pulverization markedly improved the dissolution of the β-ecdysone for increasing the cell breakage rate,and then the active ingredients could be extracted more easily.[目的]比较不同粒径露水草(Cyanotis arachnoidea C.B.Clarke)对所含β-蜕皮激素释放的影响。[方法]采用GRACEODSC18色谱柱(250mm×4.6mm,5μm),流动相甲醇:水=40:60(V/V,%)洗脱。柱温26℃,流速1.0ml/min,检测波长248nm。[结果]相同来源的露水草粉体(粒径10、30、50μm)β-蜕皮激素含量检测值和普通细粉(粒径180μm)相比,粉体中蜕皮激素激素的检测值平均高1倍多。[结论]露水草粉体中的蜕皮激素更容易溶于甲醇,其提取率明显高于普通细粉。表明露水草经过超微粉碎后,细胞破壁率到得明显提高,更有利于有效成分的溶出。

关 键 词：HPLC~ Cyanotis arachnoidea C. B. Clarke β-ecdysone 

分 类 号：S567.239[农业科学—中草药栽培]