炭疽芽孢杆菌基因芯片探针的制备  

Preparation of gene probe for Bacillus anthracis gene chip

作　　者：姜昌丽[1] 刘凌[1] 杜凌琳[2] 李云[1] 滕毅[1] 王超[2] 谭德勇[2] 王惠萱[1] 

机构地区：[1]中国人民解放军成都军区昆明总医院检验科,650032 [2]云南大学生命科学学院生物化学与分子生物学实验室,昆明650091

出　　处：《国际检验医学杂志》2011年第14期1531-1532,共2页International Journal of Laboratory Medicine

基　　金：国家高技术研究发展计划("863计划")资助项目(2006AA020902)

摘　　要：目的制备炭疽芽孢杆菌(BA)基因芯片探针,应用于BA基因芯片的检测。方法采用PCR方法,以BA疫苗株A16R基因组DNA为模板,扩增pXO1质粒上的8个特异性片段,连接至pMD19-T Simple载体,构建分别包含8个探针序列的重组质粒,以其为PCR扩增模板获取探针DNA。结果基因测序证明,成功获得BA的3个探针。结论 BA基因探针的成功获取,为炭疽芽孢杆菌基因芯片的制备奠定了基础。Objective To prepare the gene probe for Bacillus anthracis（BA） gene chip for detection of BA. Methods Eight spe- cific gene fragments were obtained by the PCR amplification of pXO1 plasmid from A16R,and inserted into pMD19-T Simple vec~ tors to construct eight recombinant plasmids. The targeting probes were amplified from these successfully constructed recombinant vectors. Results Three probe gene segments for BA gene chip were obtained. Conclusion The acquirement of gene probe for BA laid the foundation for the preparation of BA gene chip.

关 键 词：芽孢杆菌 炭疽 质粒 寡核苷酸序列分析 DNA探针 

分 类 号：R346[医药卫生—基础医学]