核酸扩增及微流芯片技术在献血者HCV筛查中的应用  

Application of HCV nucleic amplification and microfluidic chip technique in blood donors

作　　者：姚仁南[1] 姚根宏[2] 栾建凤[2] 陈复兴[1] 陈玲[1] 陈娜云[1] 

机构地区：[1]中国人民解放军第九七医院输血科,江苏徐州221004 [2]中国人民解放军南京军区南京总医院输血科,210002

出　　处：《国际检验医学杂志》2011年第14期1540-1542,共3页International Journal of Laboratory Medicine

基　　金：南京军区医学科技创新重点课题资助项目(No.08Z009)

摘　　要：目的应用核酸扩增和微流芯片分析方法,用于献血者血液标本丙型肝炎病毒(HCV)的检测。方法选取80份疑似HCV感染的血液标本,分别采用2种ELISA法、胶体金法、化学发光免疫分析(CLIA)法和核酸扩增后微流芯片法分析。结果经过核酸扩增的HCV阳性血液经分析后皆出现预期大小片段的特异性条带,而阴性血液缺少相应的条带。特异性巢式PCR扩增及微流芯片法检测的阳性率与CLIA法差异无统计学意义(P>0.05),与ELISA法和胶体金法差异有统计学意义(P<0.01)。结论特异性巢式PCR扩增及微流芯片法结果稳定、准确,灵敏度高于ELISA法,可用于血液筛查。Objective To apply nucleic amplification and microfluidic chip technique for detection of hepatitis C Virus （HCV） m blood donors. Methods The selected 80 blood samples,suspicious with HCV infection,were respectively analyzed with two differ ent ELISA reagents, colloid gold reagent, chemiluminescence immunoassay （CLIA） and reverse transcriptase-polymerase chain reac tion combined with microfluidic chip assay. Results Specific DNA zone was shown in HCV positive samples with 210 bp fragment. However, there was no corresponding DNA zone in the negative samples. Compared with CLIA, the positive rates of nucleic amplifi cation and microfluidie chip assay had no significant differences （P~〉0.05）. But compared with ELISA and colloid gold reagent, there are significant differences （P^0.01）. Conclusion The nucleic amplification and microfluidic chip assay were steady and relia ble,and more sensitive than ELISA,qualified for the future blood screening for HCV.

关 键 词：肝炎病属 化学发光测定法 酶联免疫吸附测定 核酸检测 微流芯片法 

分 类 号：R446.6[医药卫生—诊断学]