血红蛋白在二氧化钛球腔阵列上的直接电化学研究  

Direct Electrochemistry of Hemoglobin on TiO_2 Cavities Array

作　　者：周宇[1] 周丽娟[1] 孙磊[1] 尹凡[1] 

机构地区：[1]常熟理工学院化学与材料工程学院,江苏常熟215500

出　　处：《常熟理工学院学报》2011年第8期61-67,共7页Journal of Changshu Institute of Technology

基　　金：国家自然科学基金(20975073);江苏省自然科学基金(BK2008147)资助项目

摘　　要：采用Langmuir-Blodgett技术和溶胶-凝胶(sol-gel)法在氧化铟锡(ITO)表面制备了TiO2球腔阵列.研究了TiO2球腔阵列的电化学性质,结果表明:所制备的TiO2球腔阵列具有微电极阵列特性.将血红蛋白(Hb)直接吸附在TiO2球腔阵列内部,制备过氧化氢(H2O2)生物传感器.修饰电极对过氧化氢(H2O2)的电流响应快速稳定、重现性和选择性较好,在9.00×10-7～4.44×10-4mol/L范围内H2O2浓度与响应电流呈现良好的线性关系,其检出限为3.12×10-7mol/L,米氏常数为0.138mmol/L.The TiO2 cavities array was fabricated on indium-tin oxide electrode surface by using Lang-muir-Blodgett technique and sol-gel technique. The electrochemistry property of TiO2 cavities array was investigated. The result showed that TiO2 cavities array had the property of microelectrode arrays. An amperometric biosensor for detection of H202 was prepared by adsorbing hemoglobin（Hb） in TiO2 cavities directly. In pH 7.0 phosphate buffer, the CV curve of Hb/TiO2 cavities array electrode showed a pair of reversible redox peaks, the catholic and anodic peak appeared at 0.042 and 0.127V, its direct electron transfer rate was 2.95s^-1. The amperometric response of the biosensor possessed properties of speediness, good stability, reproducibility, and selectivity. Under the optimal conditions, the calibration curve was linear in the range of 9.00×10^-7-4.44×10^-4 mol/L for H2O2 concentration with a detection limit of 3.12×10^-7 mol/L and the apparent Michaelis-Menten constant for HRP modified electrode was 0.138 mmol/L.

关 键 词：二氧化钛 血红蛋白 微电极阵列 直接电化学 

分 类 号：O657[理学—分析化学]