急性心肌梗死后经冠脉移植骨髓基质干细胞对心功能的影响  

The effect on cardiac function Bone marrow stromal stem cells transplantation via coronary artery after acute myocardial infarction

作　　者：王梓良[1] 廖伟[1] 钟一鸣[1] 谢东明[1] 谢东阳[1] 

机构地区：[1]赣南医学院第一附属医院心内科,江西赣州341000

出　　处：《赣南医学院学报》2011年第4期528-530,F0003,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：目的:通过对犬骨髓基质干细胞进行分离、诱导及培养,并经冠脉移植诱导好的骨髓基质干细胞,探讨自体骨髓基质干细胞修复受损心肌的能力。方法:12只杂种犬,依手术日期随机分为两组,Ⅰ组:对照组(6只),仅移植与干细胞组等量的细胞培养液;Ⅱ组:移植组(6只),移植培养好的自体骨髓基质干细胞;结扎犬冠状动脉前降支制备急性心肌梗死模型,细胞移植组将体外分离、诱导及培养的自体骨髓基质干细胞经标记后在心肌梗死后1～2 h经冠状动脉植入心脏;对照组植入等量培养液;所有动物在手术后7天、11周分别行超声心动图检查;实验完成后取犬心脏行免疫组织化学分析。结果:骨髓基质干细胞(MSSCs)移植后11周,与对照组相比,移植组左室射血分数(LVEF)明显增加(P<0.05)。免疫组织化学检测示:移植组可以检测到5-溴脱氧尿核苷(B rdu)标记阳性细胞,对照组5-溴脱氧尿核苷(B rdu)免疫组化检测为阴性,位于5-溴脱氧尿核苷(B rdu)标记阳性细胞同一部位的连续组织切片处肌钙蛋白Ⅰ(Tropon inⅠ)染色阳性和连接蛋白43(Connexin43)染色阳性。结论:急性心肌梗死后经冠脉移植的骨髓基质干细胞(MSSCs)可改善心肌的血流灌注与代谢,改善心功能,提高左室射血分数(LVEF)。Objective：To investigate the capability of autologous bone marrow stromal stem cells for repairing of damaged myocardium through the establishment of acute myocardial infarction model and the inducement of bone marrow stromal stem cells.Methods：Twelve hybrid dogs were randomly separated into two groups including the control group（n=6） and the cell transplantation group（n=6） according to the date of surgical operation.Anterior descending coronary arteries of hybrid dogs were ligated to establish the models of acute myocardial infarction.Autologous bone marrow stromal stem cells induced and cultured in vitro were labeled and transplanted into myocardium via coronary artery 1~2 hours after acute myocardial infarction in the cell transplantation group,and the same volume of culture media was transplanted into those in the control group.Echocardiography were applied seven days and eleven weeks after operation.Then the hybrid dogs were executed and the hearts were obtained for immunohistochemical analysis.Results：Compared with control group,LVEF in cell transplantation group was significangly higher than that of control group（P0.05） 11 weeks after MSSCs implantation,the improvement of myocardial segment in cell transplantation group was higher than that of control group（P0.05）.In cell transplantation group,BrdU and Troponin I and Connexin 43 positive stain were tested with immunohistochemistry,then those were negative in control group.Conclusions：Bone marrow stromal stem cells survived and propagated in survival myocardium after transplantation via coronary artery.These cells can improve the blood perfusion and metabolism of survival myocardium,improving LVEF and myocardial function.

关 键 词：急性心肌梗死 冠状动脉 移植 骨髓基质干细胞 

分 类 号：R542.22[医药卫生—心血管疾病]