激素剂量、小鼠品系及周龄对超数排卵的影响  

The Impact of Hormone Dosage,Mice Strains and Age on Superovulation

作　　者：宋绍征[1] 王怡[1] 王宝珠[1] 杜庆辉[1] 陈则东[1] 龚文[1] 成勇[1] 

机构地区：[1]扬州大学兽医学院,扬州225009

出　　处：《实验动物科学》2011年第4期5-8,共4页Laboratory Animal Science

摘　　要：目的采用孕马血清促性腺激素(PMSG)和人促绒毛膜性腺激素(hCG)对不同品系和周龄小鼠进行超数排卵处理,比较激素(PMSG/hCG)注射剂量、小鼠品系和周龄对超排效果的影响,以便获得比较优良的超排方法。结果 1)激素剂量为PMSG(10 IU/只)+hCG(10 IU/只)的超排效果优于PMSG(5 IU/只)+hCG(5 IU/只),但无显著差异(P>0.05);2)相同剂量时I,CR小鼠和C57BL/6J小鼠的超排效果显著优于BALB/c小鼠和FVB小鼠(P<0.05);3)5周龄与8周龄的小鼠比较,8周龄超排效果较好,但差异不显著(P>0.05)。结论采用10 IUPMSG+10 IU hCG的组合对8周龄的ICR、C57BL/6J进行超排处理效果相对较好。Objective In this study,pregnant mare serum gonadotropin（PMSG） and human chorionic gonadotrophin（hCG） were used to make treatments of superovulation with different strains and ages of mice,meanwhile to obtain a better way for superovulation,the dose of hormones（PMSG/hCG）,mouse strains and weeks on the effect of superovulation were compared.Results（1） the hormone doses（with 10 IU） were better than those with 5 IU,but no significant difference was observed,（2）when the mice were injected with determined dosage of hormone,the effect of superovulation with ICR mice and C57BL/6J mice were significantly better than the BALB/c mice and FVB mice（P0.05）,（3） meanwhile,the effect of mice with 8 weeks were better than those with 5 weeks without significance（P0.05）.Conclusion In this laboratory,using the hormone doses of 10 IU PMSG ＋10 IU hCG to 8 weeks old ICR,C57BL/6J were relatively good effect of superovulation.

关 键 词：超数排卵 剂量 激素 周龄 品系 小鼠 

分 类 号：Q95-331[生物学—动物学]