家蝇幼虫抗菌肽MDL-2对细菌细胞渗透性及代谢功能影响  

Effect of Antimicrobial Peptide MDL-2 from Musca domestica Larvae on Permeability and Metabolism of Bacteria

作　　者：宫霞[1] 王兰芳[2] 乐国伟[3] 

机构地区：[1]上海商学院食品学院,上海200235 [2]同济大学基础医学院预防医学教研室,上海200092 [3]江南大学食品学院,无锡214036

出　　处：《氨基酸和生物资源》2011年第3期1-5,9,共6页Amino Acids & Biotic Resources

基　　金：上海市教委科研创新项目(09YZ470);中央级科研院所基础性专项资金工作项目重点项目(2001DEA-20022)

摘　　要：研究了家蝇幼虫抗菌肽MDL-2与细菌相互作用时,抗菌肽MDL-2对细菌细胞壁的溶解作用、细胞膜渗透性和代谢的影响。抗菌肽MDL-2在抗菌过程中首先与细菌的细胞壁相互作用,使其破裂,抗菌肽对革兰氏阴性细菌大肠杆菌细胞壁的作用有浓度依赖性,而对革兰氏阳性细菌金黄色葡萄球菌MDL-2在较低的浓度时即可发生细胞壁破坏作用;抗菌肽MDL-2与金黄色葡萄球菌的细胞膜作用曲线呈现典型的S型,存在较强的协同效应,并随着作用时间的延长细胞的渗透性增强;抗菌肽对细菌的代谢功能有较强的干扰作用,阻碍菌体的生命活动,加速菌体死亡。Effect of antimicrobial peptide MDL -2 from and metabolism of bacteria were investigated. The cell wall Musca domestica larvae on dissolution and permeability of bacteria was ruptured by interaction of antimicrobial peptide MDL - 2 with bacteria. Gram - negative bacteria E. coli had concentration - dependency when antibacterial peptide MDL - 2 assembled to certain concentration on cell outer membrane. The cell wall of Gram - positive bacte- ria S. aureus were fallen apart in lower concentration of MDL - 2. The S - type graph showed synergism while MDL -2 interplayed with cytoplasmic membrane of S. aureus. Metabolism function of bacteria was interfered with antibacterial peptide MDL- 2, so bacteria were accelerated death.

关 键 词：家蝇幼虫 抗菌肽 渗透性 β-半乳糖苷酶 代谢 

分 类 号：Q962[生物学—昆虫学]