重金属胁迫对塔胞藻细胞密度及叶绿素荧光特性的影响  

EFFECTS OF HEAVY METAL EXPOSURE ON THE CELL DENSITY AND CHLOROPHYLL FLUORESCENCE OF PYRAMIDOMONAS SP.

作　　者：王帅[1,2] 梁英[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]国家海洋局第一海洋研究所海洋生物活性物质重点实验室,山东青岛266061

出　　处：《海洋湖沼通报》2011年第3期8-20,共13页Transactions of Oceanology and Limnology

基　　金："十一五"国家科技支撑计划(2006BAD069A03)资助

摘　　要：采用调制式叶绿素荧光仪测定了重金属(Cu2+、Zn2+、Cd2+)胁迫(24h、48h、72h和96h)对塔胞藻叶绿素荧光特性的影响。测定的主要参数有:PSII的原初光能转化效率(Fv/Fm)、PSII的潜在活性(Fv/Fo)、PSII的实际光能转化效率(Yield)、相对表观电子传递效率(rETR)、光化学淬灭(qP)和非光化学淬灭(NPQ)。研究结果表明,重金属离子(Cu2+、Zn2+、Cd2+)胁迫下塔胞藻的叶绿素荧光参数Fv/Fm、Fv/Fo、Yield、rETR和qP均明显降低,并且随着重金属离子浓度的增加,胁迫时间的延长,下降幅度逐步增大;NPQ变化不规律;细胞密度和叶绿素相对含量也显著降低。对3种重金属离子的半抑制浓度(EC50)的计算结果表明,3种重金属毒性大小顺序为Cu2+>Cd2+>Zn2+。The modulate chlorophyll fluorescence kinetics of Pyramidomonas sp.under different heavy metal concentrations（Cu2＋,Zn2＋,Cd2＋） and different time（24h,48h,72h,and 96h） were investigated with Water-PAM chlorophyll fluorometer.The main parameters determined in the present study included the maximal photochemical efficiency of PSII（Fv/Fm）,the potential activity of PSII（Fv/Fo）,the actual photochemical efficiency of PSII in the light（Yield）,the relative electron transport rate（rETR）,the photochemical quenching（qP） and the non-photochemical quenching（NPQ）.The results showed that the fluorescence parameters Fv/Fm,Fv/Fo,Yield,rETR,and qP of Pyramidomonas sp.all decreased under heavy metal（Cu2＋,Zn2＋,Cd2＋） exposure.The changed degree of the above parameters increased with the increase of heavy metal concentration and increasing exposure time.NPQ changed erratically with the increasing exposure time under heavy metal exposure.The cell density and relative chlorophyll content were also decreased under heavy metal exposure.The EC50（the effective concentration causing a 50% inhibition） values indicated that the toxicity results can be expressed as Cu2＋ Cd2＋Zn2＋.

关 键 词：塔胞藻 重金属胁迫 细胞密度 叶绿素荧光特性 

分 类 号：Q945.78[生物学—植物学]