人胚肺成纤维细胞中HSP47与TGF-β_1关系的探讨  

Relation between HSP47 and TGF-β_1 in human embryonic lung fibroblasts

作　　者：汤艳[1] 郑金旭[1] 黄振杰[1] 管淑红[1] 许清[1] 刘继柱[1] 

机构地区：[1]江苏大学附属医院呼吸内科,江苏镇江212001

出　　处：《重庆医学》2011年第27期2705-2706,2708,F0002,共4页Chongqing medicine

基　　金：卫生部科研基金资助项目(wkj2006-2-026);江苏省"333工程"基金资助项目(苏人才办2007-16-09)

摘　　要：目的分析在人胚肺成纤维细胞(HELF)中热休克蛋白47(HSP47)与转化生长因子-β1(TGF-β1)之间的关系。方法将HELF分为4组:对照组、热休克组、TGF组及联合组。采用WST-8法检测HELF细胞活力和Western blot检测各组细胞HSP47、Ⅰ型胶原(ColⅠ)、α-平滑肌肌动蛋白(α-SMA)、波形蛋白(Vi mentin)表达情况。结果 TGF-β1能促进HELF的增殖(P<0.05);在TGF-β1作用下HELF中上述蛋白表达上调,在TGF-β1联合热休克反应的作用下上调更明显,而单在热休克反应后细胞HSP47表达显著,ColⅠ却未见明显增强(P>0.05)。结论 HSP47参与TGF-β1促HELF胶原合成,在肺纤维化中起着重要的作用。Objective To analyze the relationship between heat shock protein 47（HSP47） and transforming growth factor beta 1（TGF-β1） in human embryonic lung fibroblasts（HELFS）.Methods HELFS were divided into four groups：the control group,the heat shock group,TGF group and the combination group.HELF cell viability was measured by WST assay,the expressions of HSP47,Collagen-Ⅰ,α-smooth muscle actin（α-SMA） and Vimentin in cells were detected by Western blot.Results The cell viability was gradually increased after HELF being treated by TGF-β1（P0.05）;All proteins showed obvious expression in HELF induced by TGF-β1,increased more obviously induced by TGF-β1 combined with HSR.HSP47 was obviously expressed while Collagen-I was slightly expressed after HSR（P0.05）.Conclusion HSP47 may play an important role in pulmonary fibrosis as a downstream factor regulated by TGF-β1.

关 键 词：HSP47热休克蛋白质类 转化生长因子Β1 人胚肺成纤维细胞 

分 类 号：R563[医药卫生—呼吸系统]