基于碳纳米管-硫堇/苝四甲酸二酐衍生物膜/纳米金的新型癌胚抗原电流型免疫传感器的研究  

Study on the amperometric immunosensors for Carcinoembryonic Antigen based on CNTs-Thi/ PTC-NH_2/gold nanoparticle

作　　者：胡蓉[1] 杨联云 刘兵[1] 

机构地区：[1]重庆市合川区人民医院检验科,401520

出　　处：《重庆医学》2011年第27期2745-2746,2748,F0003,F0002,共5页Chongqing medicine

摘　　要：目的探讨采用碳纳米管-硫堇(CNTs-THi)/苝四甲酸二酐衍生物膜(PTC-NH2)/纳米金(nano-Au)构建的新型的高灵敏癌胚抗原(CEA)免疫传感器的性能。方法以氧化铟锡(ITO)电极为基底,在其表面首先电沉积一层纳米金,然后依次修饰CNTs-Thi、PTC-NH2、nano-Au,固载癌胚抗原特异性抗体(anti-CEA)制得新型高灵敏癌胚抗原(CEA)免疫传感器。通过循环伏安法考察电极修饰过程的电化学特性。结果该免疫电极的峰电流响应与癌胚抗原的浓度在1.0～75.0 ng/mL的范围内保持良好的线性关系,相关系数为0.991 5,检测下限为0.5 ng/mL。结论利用该方法制备的免疫传感器具有灵敏度高、线性范围宽、检测下限低等优点。Objective To explore the performance of a highly sensitive amperometric immunosensor for carcinoembryonic antigen（CEA） detection was made with carbon nanotubes-thionine（CNTs-Thi）,perylenetetracarboxylic dianhydride（PTC-NH2）and gold nanoparticle（nano-Au）.Methods Indium tin oxide（ITO） electrode was used as base,and on the surface carbon nanotubes-Thionine（CNTs-Thi） nanocomposite,3,4,9,10-perylenetetracarboxylic dianhydride（PTC-NH2）,nano-Au and anti-CEA was used.The CEA immunosensor was made.And then electrochemical property during the electrode modification process was observed by cyclic voltammetry（CV）.Results Under optimal conditions,the proposed immunosensor displayed a broader linear response to CEA,the working range was 1.0-75.0 ng/mL（r=0.9915） with a detection limit of 0.5 ng/mL.Conclusion The immunosensor made in this method exhibits high sensitivity,wider linear range,lower detection limit.

关 键 词：纳米管 碳 癌胚抗原 免疫传感器 苝四甲酸二酐衍生物膜 

分 类 号：R446.6[医药卫生—诊断学]