氨氮胁迫对马氏珠母贝免疫活性的影响  

Effects of Ammonia-N Stress on Immune Activity of Pinctada martensii ( Dunker )

作　　者：栗志民[1,2] 申玉春[2] 王淑敏[1] 李嘉威[1] 

机构地区：[1]广东海洋大学水产学院,广东湛江524025 [2]广东海洋大学南海水产经济动物增养殖广东省普通高校重点实验室,广东湛江524025

出　　处：《广东海洋大学学报》2011年第4期52-57,共6页Journal of Guangdong Ocean University

基　　金：广东省科技计划项目(2007A032600004)

摘　　要：在实验室条件下探讨氨氮胁迫对马氏珠母贝（Pinctada martensii）血细胞密度、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、溶菌酶（LSZ）、酸性磷酸酶（ACP）活性的影响。结果表明：1）不同浓度氨氮（1.25-40 mg/L）胁迫3 d后,马氏珠母贝血细胞密度随氨氮浓度的增加呈下降趋势,当氨氮浓度超过5 mg/L时血细胞密度较对照组明显下降,差异极显著（P〈0.01）;2）血清SOD活性随实验氨氮浓度（1.25-40 mg/L）的增加而明显下降,在较低氨氮浓度（1.25-5 mg/L）时,SOD活性高于对照组,氨氮浓度超过10 mg/L时,SOD活性低于对照组,各实验组SOD活性与对照组均存在极显著差异（P〈0.01）;3）当氨氮浓度为1.25-20 mg/L时,马氏珠母贝血清CAT活性随氨氮浓度的增加而升高,除氨氮1.25 mg/L组外,其余各实验组血清CAT活性较对照组极显著升高（P〈0.01）,当氨氮浓度为30-40 mg/L时,血清CAT活性呈急剧下降趋势,且低于对照组（P〈0.01）;4）马氏珠母贝血清LSZ活性随实验氨氮浓度的增加而降低,当氨氮浓度为10 mg/L以下时,血清LSZ活性高于对照组,当氨氮浓度为20 mg/L以上时,血清LSZ活性较对照组极显著降低（P〈0.01）;5）氨氮浓度为1.25-10 mg/L时,马氏珠母贝血清ACP活性随氨氮浓度的增加而升高,且高于对照组（P〈0.01）,当氨氮浓度超高20 mg/L时,马氏珠母贝血清ACP活性随氨氮浓度的增加急剧降低,且低于对照组（P〈0.01）。Effects of ammonia-N stress on immune activity of Pinctada martensii were investigated in laboratory condition.The results were as follows：（1） the density of haemocytes of Pinctada martensii decreased as increasing the concentration of ammonia-N,its being stressed in various concentration of ammonia-N（1.25-40 mg/L）for 3 days.The ammonia concentration being in excess of 5 mg/L,the density of haemocytes of Pinctada martensii was significantly lower than the control（P0.01）.（2） The activity of serum SOD of Pinctada martensii significantly lower with increasing the concentration of ammonia-N from 1.25 to 40 mg/L.However,the activity of serum SOD of Pinctada martensii significantly higher than the control（P0.01）,its being stressed in the relatively low concentration of ammonia-N（1.25-5 mg/L）,and the activity of serum SOD of that significantly lower than the control（P0.01）,the ammonia concentration being in excess of 10 mg/L.（3） The activity of serum CAT of Pinctada martensii significantly higher with increasing the concentration of ammonia-N from 1.25 to 20mg/L,which was significantly higher than the control（P0.01） except ammonia-N 1.25 mg/L group.Compared the control,the activity of serum CAT markedly lower（P0.01） with increasing the concentration of ammonia-N from 30 to 40mg/L.（4） The activity of serum LSZ of Pinctada martensii significantly reducing with increasing the concentration of ammonia-N from 1.25 to 40mg/L.the activity of serum LSZ significantly higher than the control（P0.01）,when concentration of ammonia-N being no more than 10 mg/L.and the activity of serum LSZ significantly lower than the control（P0.01）,the ammonia concentration being in excess of 20 mg/L.（5） The activity of serum ACP of Pinctada martensii significantly higher with increasing the concentration of ammonia-N from 1.25 to 10 mg/L,and its being significantly higher than the control（P0.01）.but the activity of serum ACP significantly decreased and lower than the control（P0.01） w

关 键 词：马氏珠母贝 氨氮胁迫 免疫活性:血细胞 超氧化物歧化酶 过氧化氢酶 溶菌酶 酸性磷酸酶 

分 类 号：S969.316.1[农业科学—水产养殖] Q959.215.45[农业科学—水产科学]