尼泊尔菊三七的离体快繁技术研究  

Study on Rapid Micropropagation Technique in Vitro of Medical Plant Gynura nepalensis DC.

作　　者：高倩[1] 李娜[1] 贾景明[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016

出　　处：《中国药业》2011年第18期20-22,共3页China Pharmaceuticals

摘　　要：目的依据尼泊尔菊三七野生资源开发利用情况,以尼泊尔菊三七的茎段为外植体,建立其离体快繁体系。方法通过正交设计法,添加不同浓度激素[6-苄氨基嘌呤(6-BA)和萘乙酸(NAA)]、无机元素和活性炭,建立包括丛生芽诱导、生根、壮苗以及移栽的离体快繁体系。结果用75%乙醇和0.1%升汞对外植体消毒的时间分别为20 s,6 min;尼泊尔菊三七离体快繁的最佳增殖培养条件为MS+萘乙酸0.5 mg/L+6-苄氨基嘌呤1.0 mg/L+蔗糖30 g/L+琼脂5.0 g/L,pH=6.0;壮苗生根的最佳培养条件是1/2 MS+萘乙酸0.5 mg/L+6-苄氨基嘌呤1.0 mg/L+蔗糖30 g/L+活性炭2.0 g/L+琼脂5.0 g/L,pH=6.0;移栽期间的最佳基质是腐殖土∶木炭土=3∶2。结论成功建立尼泊尔菊三七离体快繁体系,为将来大规模生产奠定了基础。Objective To establish the method of rapid micropropagation system according to the exploitative situation of Gynura nepalensis DC.wild resources for getting the best culture conditions.Methods The orthogonal methods were used to determine the optimal concentration of different hormones（6-BA and NAA） in the differentiation medium with different concentrations of inorganic elements and activated carbon to establish the rapid micropropagation system including multiple shoot clumps induction,rooting,strong seedling and transplanting.Results The sterilizing time of explants in 75%ethanol and 0.1%mercuric chloride was 20 s and 6 min respectively.The best culture conditions for rapid micropropagation were MS media supplemented with 0.5 mg/L NAA,1.0 mg/L 16-BA,30 g/L sucrose and 5.0 g/L agar.The pH value of media was adjusted to 6.0.The optimal media for rooting and strong seedling was as follows：1/2 MS media supplemented with 0.5 mg/L NAA,1.0 mg/L 6-BA,30 g/L sucrose,2.0 g/L activated carbon and 5.0 g/L agar with pH adjusted to 6.0.The best substrates of transplanting were humus and charcoal soil（3 ∶2）.Conclusion The rapid micopropagation system of Gynura nepalensis DC.is established successfully,which lays the foundation for large-scale production in future.

关 键 词：尼泊尔菊三七 离体快繁 茎段 

分 类 号：S567.236[农业科学—中草药栽培] R282.71[农业科学—作物学]