反相高效液相色谱法测定茵陈合血胶囊中黄芩苷含量  

Determination of Baicalin in Yinchen Hexue Capsule by RP-HPLC

作　　者：蒋大义[1,2] 张娓[1,2] 杨宏图[1,2] 马惠莱[1,2] 王惠玲[1,2] 常翠[1,2] 

机构地区：[1]广东省深圳市人民医院 [2]暨南大学第二附属医院药学部,广东深圳518020

出　　处：《中国药业》2011年第18期30-31,共2页China Pharmaceuticals

基　　金：深圳市科技计划项目;项目编号:200903011;广东省医院药学研究基金项目;项目编号:2011A30

摘　　要：目的建立测定茵陈合血胶囊中黄芩苷含量的反相高效液相色谱(RP-HPLC)法。方法色谱柱为Shimpak VP-ODS柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-醋酸(52∶48∶1),流速为1.0 mL/min,检测波长为280 nm,进样量为10μL,柱温为25℃。结果黄芩苷与杂质色谱峰分离良好;平均加样回收率为99.96%,RSD=0.23%(n=6);日内、日间精密度的RSD均小于5%,黄芩苷质量浓度在10.55～63.30 mg/L范围内与峰面积线性关系良好,r=0.999 8。结论方法简便、灵敏、快速、准确、重复性好,可用于茵陈合血胶囊中黄芩苷的含量测定和稳定性分析。Objective To establish a HPLC method for the determination of baicalin in Yinchen Hexue Capsule.Methods The separation of sample was carried on the Shimpak VP-ODS column（250 mm×4.6 mm,5 μm） with the mobile phase of methanol-water-acetic acid（52 ∶48 ∶1） at the flow rate of 1.0 mL/min.The detection wavelength was set at 280 nm.The sample size was 10 μL and the column temperature was 25 ℃.Results The chromatographic peaks of baicalin and impurities were separated well.The mean recovery rate of baicalin was 99.96%,RSD=0.23%（n=6）.The inter-day RSD and intra-day RSD were all less than 5%.The linearity was determined over the range of 10.55-63.30 mg/L（r=0.999 8）.Conclusion This method is simple,sensitive,rapid,accurate and reproducible,which is suitable for the quantitative determination and the stability study of baicalin in Yinchen Hexue Capsule.

关 键 词：茵陈合血胶囊 黄芩苷 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]