高山离子芥水提物对小鼠脑缺血再灌注后炎症反应的影响  

Effect of water extract of Chorispora bungeana on inflammation response following cerebral ischemia/reperfusion in mice

作　　者：孙雅煊[1,2] 王东晖[3] 刘婷[4] 戴雪伶[1] 李雨瑶[3] 李雅博[3] 杨冰玉[3] 张华[3] 安黎哲[2,3] 

机构地区：[1]北京联合大学应用文理学院,北京100191 [2]中国科学院寒区旱区环境与工程研究所,甘肃兰州730000 [3]兰州大学生命科学院,甘肃兰州730000 [4]石河子大学药学院,新疆石河子832003

出　　处：《兰州大学学报（医学版）》2011年第3期1-5,共5页Journal of Lanzhou University（Medical Sciences）

基　　金：国家杰出青年基金(30625008);国家高技术研究发展计划基金(2007AA021401);国家重点基础研究发展计划基金(2007CB108900);北京市属高等学校人才强教计划项目

摘　　要：目的观察高山离子芥水提物对脑缺血再灌注后脑组织炎症反应的影响。方法雄性ICR小鼠随机分为模型组、假手术组、高山离子芥低、中、高剂量组和依达拉奉组。建立小鼠大脑中动脉栓塞/再灌注模型。采用HE染色法观察组织病理学变化,免疫组化法测定细胞黏附分子-1表达,比色法测定髓过氧化物酶活性。结果脑缺血再灌注导致组织病理学损伤、细胞黏附分子-1表达及髓过氧化物酶活性增加。与模型组相比,高山离子芥组组织病理学变化减轻、细胞黏附分子-1表达及髓过氧化物酶活性明显降低。结论高山离子芥水提物可改善脑缺血再灌注所诱导的炎症损伤。Objective To investigate the effect of water extract of Chorispora bungeana （CB） on brain inflammatory response following cerebral ischemia/reperfusion. Methods Male ICR mice were randomly divided into model group, sham group, CB low, middle and high dosage group, and edaravone group. The mice middle cerebral artery occlusion/reperfusion model was established. HE staining method was used to observe histopathology change. Immunohistochemistry method was applied to detect intercellular adhesion molecule-1 （ICAM-1） expression and colorimetric method was adapted to assay myeloperoxidase （MPO） activity. Results Cerebral ischemia/reperfusion led to histopathology injury, the augmentation of ICAM-1 expression and MPO activity. Compared with model group, the histopathology change, ICAM-1 expression and MPO activity were obviously decreased in CB group. Conclusion The extract of CB can ameliorate the inflammatory injury induced by cerebral ischemia/reperfusion.

关 键 词：高山离子芥 细胞黏附分子-1 脑缺血再灌注 炎症反应 

分 类 号：R285.5[医药卫生—中药学]