山奈酚对人小细胞肺癌H446细胞凋亡的影响  

Effects of Kaempferol on Apoptosis of Human Small Cell Lung Cancer H446 Cells

作　　者：廖峥嵘[1] 沈永青[2] 赵娟[1] 吕品[1] 王忠海[1] 雷宇华[1] 

机构地区：[1]河北医科大学细胞生物教研室,河北石家庄050017 [2]河北医科大学西山校区,河北石家庄050200

出　　处：《河北师范大学学报（自然科学版）》2011年第5期510-514,共5页Journal of Hebei Normal University：Natural Science

基　　金：河北省科技支撑计划(08206117D);河北省医学科学研究重点课题(08259)

摘　　要：研究山奈酚诱导人小细胞肺癌H446细胞凋亡的作用及可能的作用机制.采用MTT法检测山奈酚对小细胞肺癌H446细胞增殖的抑制作用;采用DAPI染色检测经山奈酚处理后细胞凋亡的形态变化;采用RT-PCR和Western-blot技术检测山奈酚处理前后H446细胞p53,bax,bcl-2 mRNA和蛋白的表达.结果显示,不同浓度的山奈酚能抑制人小细胞肺癌H446细胞生长,且随浓度增加抑制作用增强;H446细胞在山奈酚诱导下出现典型的凋亡形态;20μmol/L以上浓度的山奈酚处理后,p53,bax mRNA和相应蛋白表达均升高,而bcl-2 mRNA和蛋白表达降低.研究表明,山奈酚对H446细胞增殖有抑制作用,并能促进其凋亡.p53,bax和bcl-2在山奈酚诱导人小细胞肺癌H446细胞凋亡中起重要作用.To investigate kaempferol induced apoptosis in small cell lung cancer cell lines H446 and its potential mechanism.MTT assay was used to determine the inhibitory rate of kaempferol with different concentrations on the proliferation of H446 cells.The cell apoptosis induced by kaempferol was detected with DAPI dye.RT-PCR and Western-blot were used to detect the changes of p53,bax and bc1-2 in mRNA and protein levels in H446 cells.MTT assay showed that the proliferation of H446 cells was markedly inhibited after treatment with kaempferol for 24 h;typical apoptotic morphology were observed in H446 cells with DAPI staining after induced by kaempferol.RT-PCR and Western-blot results indicated that the expression of p53 and bax were increased but bcl-2 was decreased by kaempferol.The result suggested that kaemperol could inhibit the proliferation of H446 cells and induce part of cells to apoptosis.p53,bax and bc1-2 may contribute to the apoptosis mechanism.

关 键 词：山奈酚 人小细胞肺癌H446 p53 BAX BCL-2 细胞凋亡 

分 类 号：Q3[生物学—遗传学]