青钱柳种子不同部位发芽抑制物质的测定  

Evaluation on germination inhibitors in different fractions of Cyclocarya paliurus seeds

作　　者：尚旭岚[1] 孙容 徐锡增[1] 方升佐[1] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]重庆金佛山国家级自然保护区管理局

出　　处：《林业科技开发》2011年第5期29-32,共4页China Forestry Science and Technology

基　　金：国家林业公益性行业科研专项(编号:200904046);江苏省高等学校创新团队科研计划(编号:苏教科[2009]10号);江苏省"六大人才高峰"高层次人才项目

摘　　要：对青钱柳种子不同部位乙醚提取液进行了生物测定并对果皮和种皮提取液进行GC-MS鉴定,结果表明:青钱柳种子不同部位乙醚提取液的抑制强度为果皮>种皮>种胚;果皮和种皮乙醚提取液的GC-MS鉴定结果显示,青钱柳种子中存在庚酸、辛酸、棕榈酸和邻苯二甲酸二乙酯等发芽抑制物质。对邻苯二甲酸二乙酯和棕榈酸标准品进行生物测定,确定其半抑制浓度(IC50)分别为478.63μL/L和602.56mg/L。The inhibitory effects of ether extracts from different fractions of Cyclocarya paliurus seeds were evaluated using the method of bioassay, and then the inhibitors of pericarp and seed coat were identified by gas chromatography-mass spec- troscopy （GC-MS）. The bioassay results indicated that the inhibitors were distributed in all the fractions of the seeds, and the following activity series was obtained： pericarp 〉 seed coat 〉 embryo. The GC-MS identification results showed that there existed several inhibitors such as heptanoic acid, octanoic acid, hexadecanoic acid and diethyl phthalate. The ICso （Inhibitory concentration 50% ） of two standard substances including diethyl phthalate and hexadecanoic acid were 478.63 μl/L and 602.56mg/L respectively.

关 键 词：青钱柳种子 发芽抑制物质 邻苯二甲酸二乙酯 棕榈酸 

分 类 号：S567.53[农业科学—中草药栽培]