楸树不同类型胚性和非胚性愈伤组织的细胞学观察  

Morphological and cytological observation on the different callus in Catalpa bungei

作　　者：任莺 彭方仁[2] 杨燕[2] 王列富[3] 

机构地区：[1]南京市林业站,南京210036 [2]南京林业大学森林资源与环境学院 [3]信阳职业技术学院

出　　处：《林业科技开发》2011年第5期43-45,共3页China Forestry Science and Technology

基　　金：国家"十一五"科技支撑项目(编号:2006BAD24B08);江苏林业三项工程项目[编号:lysx(2009)45];河南省重点科技攻关项目(编号:092102110111)

摘　　要：以楸树幼嫩茎段为外植体,接种在诱导培养基上诱导初始愈伤组织,以初始愈伤组织诱导胚性和非胚性愈伤组织,分别利用扫描电镜和透射电镜对愈伤组织进行了观察。结果表明:不同类型愈伤组织在形态学和细胞学特性方面存在显著差异。III型白色愈伤组织I、V型褐色愈伤组织为非胚性愈伤组织;I型绿色愈伤组织、II型紫色愈伤组织可能为楸树胚性愈伤组织。为楸树体细胞胚胎发生提供了理论基础。The stem segments of Catalpa bungei were taken as explants and cultured on inducing culture medium to induce the embryyogenic and non-embryogenic callus. The results showed that there were four kinds of callus observed by the scan- ning electron microscopy and the transmission electron microscopy. The white type III and the brown type IV callus were non-embryogenic callus, while the green type I and the purple type II callus were likely to be embryogenic callus.

关 键 词：楸树 愈伤组织 体细胞胚 细胞学 

分 类 号：S791.22[农业科学—林木遗传育种]