黑莓品种‘Black Butte’的组织培养  

Tissue culture of a blackberry cultivar 'Black Butte'

作　　者：胡淑英[1] 王小敏[1] 吴文龙[1] 李维林[1] 

机构地区：[1]江苏省.中国科学院植物研究所南京中山植物园,南京210014

出　　处：《林业科技开发》2011年第5期107-110,共4页China Forestry Science and Technology

基　　金：国家自然科学基金项目(编号:31000753);江苏省科技基础设施建设计划项目(编号:BM2009041);江苏省科技基础设施建设计划项目(编号:BM2010458)

摘　　要：以黑莓品种‘Black Butte’的带芽茎段为外植体进行组织培养研究。结果表明,基本培养基的成分、激素种类和浓度对‘Black Butte’不定芽增殖和生长均有显著影响。综合分析认为,较适基本培养基是添加0.05mmol/L铁盐的MS培养基,较适增殖培养基为MS+0.05 mmol/L铁盐+0.5 mg/L 6-BA+0.3 mg/L IBA,较适生根培养基为1/2 MS+0.05 mmol/L铁盐+0.01 mg/L NAA。The stem segments with auxiliary buds of ＇ Black Butte＇ were cultured as explants. The results showed that the effects of the chemical composition, hormone and concentrations of minimal medium on shoot proliferation and growth were significant . It was concluded that the optimum medium for growth of explants was MS ＋ 0.05 mmol/L molysite, the opti- mum medium for proliferation was MS ＋ 0. 05 mmol/L molysite ＋ 0. 5 mg/L 6-BA ＋ 0.3 mg/L IBA, the optimum medium for rooting of transferring plantlets was 1/2 MS ＋ 0.05 mmol/L molysite ＋ 0. 01 mg/L NAA.

关 键 词：黑莓 'Black Butte’ 组织培养 基本培养基 激素 

分 类 号：S663.2[农业科学—果树学]