基于线粒体Cyt b基因的黄海南部和东海银鲳群体遗传结构分析  

POPULATION GENETIC STRUCTURE OF PAMPUS ARGENTEUS IN THE SOUTH YELLOW SEA AND EAST CHINA SEA BASED ON THE MITOCHONDRIAL CYT B SEQUENCE

作　　者：赵峰[1] 庄平[1] 章龙珍[1] 施兆鸿[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源与生态重点开放实验室,上海200090

出　　处：《水生生物学报》2011年第5期745-752,共8页Acta Hydrobiologica Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金(东海水产研究所2007Z02)资助

摘　　要：采用线粒体DNA细胞色素b基因片段为遗传标记,对黄海南部和东海银鲳(Pampus argenteus)群体的遗传结构进行了分析。在所分析的6个采样点116个个体中,共检测到40种单倍型。6个群体均呈现出高单倍性多样性(0.647—0.895)和低核苷酸多样性(0.0008—0.0026)的特点。单倍型邻接关系树的拓扑结构简单,未呈现明显的地理谱系结构。分子方差分析和Fst显示银鲳的遗传变异来自群体内个体间,而群体间无显著遗传分化。Exact检验表明单倍型在两两群体间分布频率的差异不显著。研究结果表明,黄海南部、东海银鲳群体间具有高度的基因交流,是一个随机交配群体。较强的扩散能力,黄海、东海的海洋环流以及近期的群体扩张可能是造成黄海南部、东海银鲳群体间遗传同质性较高的原因。Silver pomfret（Pampus argenteus） is an important fisheries resource in China,and distributes along the coast from the Bohai Sea to the South China Sea.The fish population has been greatly decreased these years due to over ex-ploitation and the lack of the long-run and effective administer.In order to protect the fish population,it is necessary to study genetic structure of the fish population,which is very important for harvest management and stock conservation.Fish specimens（totally 116 individuals） used in this study were collected from 6 sampling locations in the south Yellow Sea and East China Sea,i.e.,Lianyungang（LYG）,Lüsi（LS）,Zhoushan（ZS）,Dongtou（DT）,Xiapu（XP） and Dongshan（DS）.The mitochondrial cytochrome b（Cyt b） gene sequences of the fish was amplified by polymerase chain reaction（PCR） technology and sequenced with ABI 3130 sequencer.One hundred and sixteen sequences were analyzed by using some softwares,viz.,Clustal X 1.83,DnaSP 5.1,MEGA 3.1,Arlequin 3.1 and Network 4.5.The results were as follows： Every specimen sequence was 1118 bp for genetic diversity analysis of the fish.Both 47 mutations of nucleotide acids without inserting or deleting one and 40 haplotypes were found among 116 individuals from 6 sampling sites.About 27.5% of the 40 haplotypes presented at least in two or more geographic groups and every one of 29 haplotypes just appeared in single one of the 6 groups.A shallow topology of haplotype neighbor-joining（NJ） tree showed no phylogeographic structure in six populations.The median-joining（MJ） network for 6 geographic popu-lations indicated that only one haplotype（Hap 1） was shared by the majority of these populations,and no significant geographic structure among populations studied.All 6 populations were characterized by high haplotype diversity（H=0.647—0.895） and low nucleotide diversity（？=0.0008—0.0026）.The ranges and the level of average genetic dis-tance were 0.00080—0.00262 and 0.00099—0.00219 for silver pomfret

关 键 词：银鲳 细胞色素B 野生群体 遗传多样性 

分 类 号：Q346[生物学—遗传学]