基于线粒体D-loop区比较分析野生与养殖斜带髭鲷种群的遗传多样性  

GENETIC DIVERSITY EVALUATION BY THE COMPARATIVE ANALYSIS ON MITOCHONDRIAL D-LOOP AREA BETWEEN WILD AND CULTURED POPULATIONS OF HAPALOGENYS NITENS

作　　者：陈竹[1] 钟山[1] 罗大极[1] 杨国华[1] 梁日深[2] 邹记兴[2] 

机构地区：[1]武汉大学遗传学系,武汉430071 [2]华南农业大学动物科学学院,广州510642

出　　处：《水生生物学报》2011年第5期761-767,共7页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金项目(编号:3077165230972258)资助

摘　　要：采集广东省深圳市南澳区野生斜带髭鲷Hapalogenys nitens(Richardson)和养殖斜带髭鲷各25个样本。通过设计特异性引物,采用PCR技术对该50个个体的mtDNA D-loop区全序列进行扩增,测序得到的序列进行比对,发现该50个序列长度变异较小,均在788—790 bp之间,野生型和养殖型区别不大。采用MEGA(version 4.0)和DnaSP(version 4.0)软件对序列进行分析,结果显示:50条序列中T、C、A和G碱基平均含量分别为29.9%、21.5%、35.2%和13.5%,其中A+T的含量(65.1%)显著高于G+C含量(34.9%),表现了明显的碱基偏倚。50个个体表现为26种单倍型,包括91个多态位点,单倍型间平均遗传距离为0.024,单倍型多态性(h)为0.958,核苷酸多态性(π)值为0.02277。研究表明,mtDNA D-loop区可以作为检测野生与养殖斜带髭鲷群体遗传多样性的有效分子标记,而广东省深圳市南澳区野生斜带髭鲷种群遗传多样性已经下降至中等水平。研究提示野生斜带髭鲷种群遗传多样性不容乐观,资源调查、种质维护与遗传评价有待深入。Hapalogenys nitens is a kind of delicious marine fish,mainly distributes in China,Japan and Korea,which has become an important culture fish in China.In recent years,as economic activity is becoming more frequent,fewer and fewer natural nitens could be captured in Guangdong,Fujian,Zhejiang and other provinces along the coast.It is important to analyze the population genetic structure of Hapalogenys nitens.For evaluating the genetic diversity of Hapalogenys nitens,50 individuals of Hapalogenys nitens were collected from Guangdong province.All of the 50 indi-viduals were come from two groups which were wild and cultured groups.Half of them was the wild population,and the other half was the cultured population.Study on genetic diversity evaluation of fish is mainly concentrated on gene mu-tation in nucleus.But in recent years,the role of mitochondrial DNA（mtDNA） mutation in genetic diversity research has been given more attention.Mitochondrial DNA（mtDNA） is known for high mutation rates caused by lack of pro-tective histones,inefficient DNA repair systems,and continuous exposure to mutagenic effects of oxygen radicals.So the analysis of mitochondrial DNA has been a potent tool in our understanding of Hapalogenys nitens genetic diversity between wild and cultured populations.The D-loop occurs in the main non-coding area of the mitochondrial DNA molecule,a segment called the control region or D-loop region.In this study,we chose the mtDNA D-Loop region for genetic diversity evaluation and examined the frequency of mutations in the mtDNA D-Loop region in 50 individuals.The mitochondrial DNA of them were extracted,and the mtDNA D-Loop region of 50 individuals were sequenced for the comparative analysis.Sequences length of the D-loop area was from 788 bp to 790 bp in 50 Hapalogenys nitens individuals by PCR amplification and electrophoresis.The sequences were analyzed by using the MEGA（version 4.0） and DnaSP（version 4.0） software.The result showed that the average content of T,C,A and G was 29.9%,21.5%,35

关 键 词：斜带髭鲷 线粒体基因组 D-LOOP 基因多态性 

分 类 号：Q346.5[生物学—遗传学]