胰岛素样生长因子结合蛋白3在宫内生长受限儿中的甲基化研究  

Methylation of insulin-like growth factor binding protein 3 gene in neonates with intrauterine growth restriction

作　　者：苏爱玲[1] 蒋犁[1] 葛芹玉[2] 

机构地区：[1]东南大学附属中大医院儿科,江苏南京210009 [2]东南大学生物电子学国家重点实验室,江苏南京210096

出　　处：《中国当代儿科杂志》2011年第9期700-703,共4页Chinese Journal of Contemporary Pediatrics

摘　　要：目的探讨胰岛素样生长因子结合蛋白3(IGFBP3)启动子区甲基化状态在胎儿宫内生长受限(IUGR)中的作用。方法选取IUGR新生儿50例及正常新生儿30例,应用甲基化特异性PCR(MSP)及高分辨率溶解(HRM)技术检测外周血中IGFBP3基因的甲基化状态。结果 IUGR组中IGFBP3启动子区完全甲基化比例为4%(2/50),部分甲基化比例为40%(20/50),未甲基化比例为56%(28/50);对照组中部分甲基化比例为13%(4/30),未甲基化比例为87%(26/30),两组甲基化率差异有统计学意义(P<0.01)。结论Objective To study the role of promoter methylation of insulin-like growth factor binding protein 3（IGFBP3） in intrauterine growth restriction（IUGR）. Methods Fifty neonates with IUGR and 30 healthy neonates were enrolled.The promoter methylation status of IGFBP3 in peripheral blood was evaluated by methylation-specific PCR（MSP） and high resolution melting（HRM） techniques. Results The complete methylation rate,partial methylation rate and non-methylation rate of IGFBP3 promoter in the IUGR group was 4%（2/50）,40%（20/50） and 56%（28/50）,respectively.The partial methylation rate and non-methylation rate of IGFBP3 promoter in the control group were 13%（4/30） and 87%（26/30）,respectively.There were significant differences in the promoter methylation rate of IGFBP3 between the two groups（P0.01）. Conclusions The promoter methylation of IGFBP3 gene is associated with the pathogenesis of IUGR.

关 键 词：胎儿宫内发育受限 胰岛素样生长因子结合蛋白3 甲基化 新生儿 

分 类 号：R714.5[医药卫生—妇产科学]