紫草素对IL-17刺激HaCaT细胞分泌VEGF影响的研究  

The research of that Shikonin effects on VEGF production in IL-17-stimulated HaCaT cells

作　　者：行敏[1] 耿龙[1] 任宏伟[1] 屈慧明[1] 王雪[1] 季永智[1] 魏仲香[1] 周鸿波[1] 

机构地区：[1]中国医科大学附属第一医院皮肤性病科,沈阳110001

出　　处：《中华微生物学和免疫学杂志》2011年第8期685-688,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（30872277）;教育部长江学者和创新团队发展计划项目（IRT0760）;辽宁省教育厅创新团队项目（2008T197）

摘　　要：目的观察白细胞介素-17（IL-17）对HaCaT细胞分泌血管内皮生长因子（VEGF）的影响及紫草素的干预作用。方法收集IL-17刺激组和紫草素处理后HaCaT细胞及其培养上清，分别用双抗体夹心酶联免疫吸附法（ELISA）及实时荧光免疫定量-聚合酶链反应法（RT-PCR）检测VEGF含量。应用细胞计数盒-8（CCK-8）检测各组HaCaT细胞活力。结果与对照组相比，不同时间IL-17刺激组HaCaT细胞及其培养上清中VEGF表达均高于对照组（P〈0．001）；紫草素处理组HaCaT细胞及其培养上清VEGF表达均低于IL-17处理组（P〈0．001）；与对照组比较，CCK-8检测各组HaCaT细胞活力无明显差异（P〉0．05）。结论IL-17可促进HaCaT细胞分泌VEGF，呈时间依赖型，而紫草素对其具有抑制作用。Objective To investigate whether IL-17 could stimulate the vascular endothelial growth factor （VEGF） production on HaCaT cells alone. We also investigated whether shikonin could inhibi- ted the proinflarnation effects of interleukin-17 （IL-17） acting on HaCaT cells. Methods We examined the expression of VEGF by double antibody sandwich enzyme-linked immunosorbent assay （ELISA） and real- time polymerase chain reaction（RT-PCR） in HaCaT cells and the cell supernatant. The viability of HaCaT cells in the drug group was detected by the Cell Counting Kit-8 （ CCK-8 ）. Results The expression of VEGF in different time IL-17-stimulated groups on HaCaT cells and the cell supernatant were higher than the con- trol group（P〈0.001 ）. The expression of VEGF in different drug treatment groups on HaCaT cells and the cell supernatant were lower than the stimulated group by IL-17 （ P〈0. 001 ）. The cell viability of different drug treatment groups have no significant difference（P〉0.05）. Conclusion We show that IL-17 specific- ally and time-dependently augmented and induced VEGF expression on HaCaT cells and the cell supernatant Then shikonin markedly inhibited the increase tengency of IL-17 effection on HaCaT cells and the cell supernatant level.

关 键 词：银屑病 HACAT细胞 IL-17 VEGF 紫草素 

分 类 号：R285[医药卫生—中药学]