EV71感染患儿Toll样受体表达初探  

Expression of pattern recognition receptor in patients of EVT1 infection

作　　者：郭建群[1] 李成荣[1] 祖莹[1] 王国兵[1] 何颜霞[1] 付丹[1] 

机构地区：[1]深圳市儿童医院儿科研究所分子免疫室,518026

出　　处：《中华微生物学和免疫学杂志》2011年第8期729-734,共6页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨肠道病毒71型（EVT1）感染患儿免疫活性细胞模式识别受体（patternrec—ognitionreceptor，PRR）及细胞因子水平的变化。方法Ev71感染患儿71例，其中轻症EV71感染组20例，重症EV71感染组25例，危重症EV71感染组26例，同年龄正常对照组20例。采用real．timePCR检测外周血单个核细胞维甲酸蛋白Ⅰ（retinoic acidinduciblegene I，RIG—I）、黑色素瘤分化相关基因5（melanoma differentitation-associated gene5，MDA5）及细胞因子（IL-12、IFN-α）表达；流式细胞术检测外周血单核／巨噬细胞（monocyte／macrophages，MC）、髓样树突状细胞（myloiddentriticcells，mDC）及浆样树突状细胞（plasmacytoiddentriticcells，pDC）Toll样受体（TLRs）表达率；ELISA检测细胞因子IL-12及IFN-α的变化。结果（1）轻症患儿仅TLR7升高，重症EV71感染患儿外周血MC、mDC、pDCTLR7表达明显升高（P〈0．05），MC、mDC高表达TLR2、3、4，危重症患儿呈下降趋势。（2）EV71感染患儿胞内模式识别受体RIG—I／MDA5mRNA表达明显增加；（3）轻症患儿DC相关细胞因子有上升趋势，重症患儿DC相关细胞因子IL-12、IFN-α明显增高（P〈O．05），轻症及危重症患儿明显降低（P〈0．05）。结论TLR7可能是免疫活性细胞识别EV71的主要模式识别受体；RIG-I／MDA5也可能参与识别EV71；合并细菌感染或细胞破坏释放的内源性配体导致TLR2或TLR4活化，诱导炎症反应。Objective To investigate the expression of pattern recognition receptor （PRR） of immune cells from the patients with enterovirus 7 1 （ EV 7 1 ） infection and the changes of cytokines. Methods Seventy-one patients and 20 age-matched healthy children were enrolled in the study. The patients were divided into three groups according to the critical degree： 20 mild patients, 25 severe patients and 26 critical patients. Real-time PCR were used to evaluate the levels of retinoic acidinducible gene I （ RIG- I ）, melanoma differentitation-associated gene 5 （MDAS）and cytokines 1L-12, IFN-α mRNA expression in peripheral blood mononuclear cells（PBMC）. The proportions of Toll like receptors（TLRs） on monocytes/macrophages （MC） , myloiddentritic cells （mDC） and plasmacytoiddentritic cells （pDC） were analyzed by flow cytometry. The concentrations of plasma cytokines （IL-12, IFN-α） were measured by ELISA. Results （1）The proportion of TLR7 is the unique TLR which is increased in mild patients and it was significantly elevated in MC, mDC and pDC in severe group （P〈0.05）, TLR2, TLR3 and TLR4 also had an enhanced expression in MC and mDC. The enhanced expression of TLRs mentioned above had a decreased trend in critical pa- tients. （2）Transcription levels of RIG-I and MDA5 was significantly elevated in EV71 infected children. （3）The expression levels of DC-associated cytokines gene （IL-12 and IFN-α） have an increased trend in mild cases and these cytokines were remarkably increased in severe patients （P〈0.05）, whereas decreased in critical cases （P〈0.05）. Conclusion TLR7 might be the main PRR recognizing EV71 in immune cells and RIG-I/MDA5 might also take part in the recognition of EVT1. The exogenous or endogenous ligands released from bacterial infection and cell destruction could result in the activation of TLR2 or TLR4, inducing the inflammatory response.

关 键 词：EV71感染 模式识别受体 TOLL样受体 RIG-I/MDA5 免疫功能 

分 类 号：R725.1[医药卫生—儿科]