不同牵伸强度对体外人肌腱细胞磷脂酶A2／环氧合酶表达的影响  

Effect of different cyclic stretching strengths on expressions of phospholipase A2 and cyclooxygenase in human tenocytes in vitro

作　　者：谢美明[1] 唐康来[1] 邓银栓[1] 李辉[1] 周建波[1] 谭晓康[1] 陈磊[1] 许建中[1] 

机构地区：[1]第三军医大学附属西南医院骨科,全军矫形外科中心,重庆400038

出　　处：《中华创伤杂志》2011年第9期822-826,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金面上资助项目（30872620）;国家自然科学基金面上资助项目（81071464）;重庆市自然基金重点资助项目（CSTC2011BA5010）

摘　　要：目的探讨不同循环牵伸强度对体外人肌腱细胞磷脂酶A2（phospholipase A2，PLA2）和环氧合酶（cyclooxygenase，COX）表达的影响。方法取4～6代人肌腱细胞在0．5Hz、4h条件下施加不同牵伸强度（4％、8％、12％），未牵伸组设为对照组。静置4h后，用RT—PCR、Western blot检测胞浆性PLA2（cytosolic PLA2，cPLA2）、COX1、COX2的表达，ELISA测定分泌性PLA2（secretory PLA2，sPLA2）的表达。结果对照组、4％、8％、12％牵伸组ePLA2、COX1、COX2基因表达随牵伸强度增加呈递增趋势，4％牵伸组cPLA2、COX1蛋白表达与对照组比较增加均不明显，差异无统计学意义（P〉0．05）；8％和12％牵伸组cPLA2及COX1蛋白表达与对照组比较明显增加，差异有统计学意义（P〈0．01）。4％、8％、12％牵伸组COX2与对照组比较，差异均有统计学意义（P〈0．01），且随牵伸强度增加而逐渐增加。sPLA2在4％牵伸组分泌量和对照组比较，差异无统计学意义（P〉0．05），8％和12％牵伸组增加明显（P〈0．01）。结论体外人肌腱细胞PLA2、COX1、COX2的表达与牵伸强度呈正相关，PLA2／COX调节系统可能成为防治肌腱病的新分子靶标。Objective To investigate the effect of different cyclic strengths on expressions of phospholipase A2 （PLA2） and cyclooxygenase （COX） in human tenocytes. Methods Human teno-cytes were uniaxially stretched with different stretching intensity （4%, 8% and 12% ） under 0.5 Hz for four hours. Nonstretched tenocytes were applied to the control group. The expressions of cytosolic PLA2 （cPLA2）, COX1 and COX2 were measured by Western blot and RT-PCR. The secretion of secretory PLA2 （sPLA2） was measured by ELISA. Results The mRNA expressions of cPLA2, COX1 and COX2 in control group, 4% , 8% and 12% stretch groups showed an increase trend. But protein expressions of cPLA2 and COX1 in 4% stretch group were increased insignificantly compared with the control group （P 〉 0.05 ）. Protein expressions of cPLA2 and COX1 in 8% and 12% stretch groups were increased more significantly compared with the control group （ P 〈 0.01 ）. The COX2 expression in 4%, 8% and 12% stretch groups showed statistical difference compared with that in the control group （ P 〈 0.01 ） and the difference increased with stretch intensity. There was no different expression of sPIA2 between 4% stretch group and control group （P = 0. 260）. However, expression of sPLA2 was increased markedly in 8% and 12% stretch groups （ P 〈 0.01 ）. Conclusions The expressions of human tendnocytes PLA2, COX1 and COX2 in vitro are positively correlated with stretch intensity. PLA2/COX system may be a new molecule target in clinical treatment of tendinopathy.

关 键 词：肌腱病 磷脂酶A2 环氧合酶 机械拉伸 

分 类 号：R6[医药卫生—外科学]