Ⅱ型胶原海绵修复兔膝关节软骨缺损的MRI与组织学对比分析  

Repair of rabbit knee cartilage defects with type Ⅱ collagen sponges： a comparison between MRI and histology

作　　者：杨小红[1] 陈鸿辉[1] 廖鹰扬[1] 曾旭文[2] 梁佩红[1] 康宁[1] 梁治平[2] 谭见容[1] 梁伟国[1] 

机构地区：[1]暨南大学医学院第四附属医院,广州市创伤外科研究所,广州510220 [2]暨南大学医学院第四附属医院放射科

出　　处：《中华创伤杂志》2011年第9期827-833,共7页Chinese Journal of Trauma

基　　金：广州市科技攻关资助项目（200623-E0371）

摘　　要：目的建立兔膝关节软骨损伤动物模型，评估自行研制的Ⅱ型胶原海绵修复关节软骨缺损的效果。方法根据既往方法研制成Ⅱ型胶原，利用胶原的自发荧光特性测试其孔径大小；将材料移植到软骨缺损动物模型上，以随机分组对照的方式进行动物实验。通过MRI联合组织病理学HE、番红O、天狼猩红偏振光染色及新生软骨面积测定、Ⅱ型胶原免疫组化染色观察软骨缺损修复状况。结果Ⅱ型胶原海绵自发荧光分层扫描显示其孔径为（93．26±38．40）μm，较接近最适软骨生长的孔径。通过横向比较动物实验同一时相点各组MRI对照组织学结果可以看出，Ⅱ型胶原海绵组在6周时首先出现零星、孤立的软骨信号，而番红O染色和免疫组化的结果证实新生的软骨同时具有一定的分泌糖胺多糖和Ⅱ型胶原基质的功能。天狼猩红偏振光测定结果显示，Ⅱ型胶原海绵组新生软骨面积远高于对照组（P〈0．01）。结论通过改进方法提取纯化的Ⅱ型胶原，无论组织病理还是MRI的结果均显示对兔膝关节软骨缺损的修复有显著效果。Objective To establish rabbit knee joint cartilage injury models to evaluate effects of the type Ⅱ collagen sponge in repair of the articular cartilage. Methods The type Ⅱ collagen sponge was prepared according to previous method and the pore size of the sponges was measured based on the collagen autofluorescence characteristics. The type Ⅱ collagen sponge was transplanted into the injury lesions of the animal model for experimental study. The regeneration of the cartilage defects was observed by using MRI, histologic HE staining, Safranin O, sirius red polarized light staining, areas determination of the newly grown cartilage and immunohistochemistry of type Ⅱ collagen. Results Autofluorescent images of confocal microscope layer scanning showed that the pore size was （93.26±38.40）μm in diameter, suitable for chondrocyte growth. Comparison between MRI and H＆E staining results showed quicker effusion absorption in the treatment groups than that in the control group, while the level of inflammatory response in the treatment group was lower than that in the control group. The sporadic cartilage signals first appeared at the 6th week. The newly formed cartilage with the expression of glycosaminoglycan and type Ⅱ collagen matrix was confirmed by Safranin O staining and immunohistochemical analysis. The sirius red polarized light staining showed that areas of the newly formed cartilage were significantly larger in the treatment group than that in the control group （ P 〈0.01 ）. Conclusion The type Ⅱ collagen sponge developed from purification can effectively repair the damaged cartilage tissues of the rabbit knee joints, as has been verified either by MRI or histology.

关 键 词：胶原Ⅱ型 软骨 关节 磁共振成像 组织学 

分 类 号：R6[医药卫生—外科学]