外源性IL-2对小鼠CD4^+CD25^+调节性T细胞影响的实验研究  

Impact of exogenous IL-2 the CD4^+CD25^+ regulatory T cells of mice

作　　者：姜晓峰[1] 郭大伟[1] 朱磊[1] 崔哲铭[1] 孙文郁[1] 林琳[1] 王学范[1] 唐裕福[1] 梁健[1] 

机构地区：[1]中国医科大学附属第四医院普外科,辽宁沈阳110032

出　　处：《中国现代普通外科进展》2011年第8期594-597,共4页Chinese Journal of Current Advances in General Surgery

基　　金：辽宁省教育厅高等学校科研资助项目(2008824)

摘　　要：目的:探讨IL-2对CD4+CD25+调节性T细胞(Tregs)的增殖及功能的影响。方法:提取B6小鼠脾脏细胞,流式细胞仪分离CD4+CD25+Tregs,将新鲜分离的CD4+CD25+Tregs与抗CD3单克隆抗体、同种同系抗原递呈细胞(APCs)及外源性IL-2共同培养,测定其增殖活性;并检测体外扩增后的CD4+CD25+Tregs的免疫抑制活性及其Foxp3的表达。结果:与外源性IL-2共同培养的CD4+CD25+Tregs增殖程度强烈,与对照组比较,差异有统计学意义(P<0.05);体外扩增的CD4+CD25+Tregs抑制CD4+CD25-T细胞增殖活性的能力与新鲜分离的CD4+CD25+Tregs相似(P>0.05)。体外扩增的CD4+CD25+Tregs的Foxp3表达与新鲜分离的CD4+CD25+Tregs亦相似(P>0.05)。结论:外源性IL-2能够消除CD4+CD25+Tregs的无反应状态,且体外扩增的CD4+CD25+Tregs保持了其抑制活性。Objective： To examine the effection of IL-2 on the proliferation and function of CD4＋CD25＋ regulatory T cells.Methods： The CD4＋CD25＋ T cells were isolated from B6 mice spleens using FACS.The freshly isolated CD4＋CD25＋ T cells were cultured with anti-CD3 monoclonal antibody（mAb）,syngeneic antigen presenting cells（APCs） and IL-2.Poliferative responses were measured by thymidine incorporation.The suppressive capability and the Foxp3 expression of in vitro-expanded CD4＋CD25＋ T cells was also investigated.Results： Exogenous IL-2 restored responsiveness of CD4＋CD25＋ T cells to anti-CD3 antibody.In vitro-expanded CD4＋CD25＋ T cells suppressed the proliferation of CD25-CD4＋ T cells similar to freshly isolated CD4＋CD25＋ T cells.The Foxp3 expression of in vitro-expanded CD4＋CD25＋ T cells was also similar to freshly isolated CD4＋CD25＋ T cells.Conclusion： Exogenous IL-2 could restore the proliferation of anergic CD4＋CD25＋ regulatory T cells.Furthermore,in vitro-expanded CD4＋CD25＋ regulatory T cells retain their potent suppressive activity.

关 键 词：CD4+CD25+调节性T细胞 白介素2 免疫抑制 

分 类 号：R392[医药卫生—免疫学]