Synthesis and characterization of arginine-modified and europium-doped hydroxyapatite nanoparticle and its cell viability  

纳米羟基磷灰石的精氨酸表面修饰与铕掺杂及其细胞活性(英文)

作　　者：赵颜忠[1,2,3] 朱军[2] 朱晒红[2,3] 黄艳艳[1] 李志友[2] 周科朝[2,3] 

机构地区：[1]中南大学湘雅三医院医学实验中心,长沙410013 [2]中南大学粉末冶金国家重点实验室,长沙410083 [3]中南大学医用材料与器械研究中心,长沙410013

出　　处：《Transactions of Nonferrous Metals Society of China》2011年第8期1773-1778,共6页中国有色金属学报（英文版）

基　　金：Project (81071869) supported by the National Natural Science Foundation of China; Project (2009637526) supported by China Scholarship Council (CSC Program);Project (2010QZZD006) supported by the Key Program of Central South University Advancing Front Foundation

摘　　要：The arginine-modified and europium-doped hydroxyapatite nanoparticles（HAP-Eu） were synthesized by hydrothermal synthesis.The prepared nanoparticles were characterized by transmission electron microscopy（TEM）,X-ray diffractometry（XRD）,Fourier transform infrared（FTIR） and zeta potential analyzer.The cell viability of HAP-Eu was tested by image flow cytometry.The results indicated that HAP-Eu is short column shapes and its size is approximately 100 nm,its zeta potential is about 30.10 mV at pH of 7.5,and shows no cytotoxicity in human epithelial cells and endothelial cells.采用水热合成法制备经精氨酸表面修饰和铕掺杂的羟基磷灰石纳米颗粒(HAP-Eu)。利用透射电镜(TEM)、Zeta电位分析仪、傅立叶红外光谱仪和X射线衍射(XRD)对HAP-Eu的成分、形貌、结构、晶粒粒径和Zeta电位进行表征,并通过成像流式细胞仪研究细胞活性。结果表明:制备的HAP-Eu粒径较均匀,约为100nm,特征峰明显;在pH=7.5时,颗粒的表面净电荷均值约为30.10mV;在人上皮细胞和内皮细胞中无毒性。

关 键 词：HYDROXYAPATITE ARGININE EUROPIUM hydrothermal synthesis cell viability 

分 类 号：TB383.1[一般工业技术—材料科学与工程]