灿烂弧菌的疏水性和生物被膜形成能力  

Cell surface hydrophobicity and biofilm formation ability of Vibrio splendidus

作　　者：李华[1] 王扬[1] 李强[1] 乔帼[1] 

机构地区：[1]大连海洋大学农业部海洋水产增养殖学重点开放实验室,辽宁大连116023

出　　处：《中国水产科学》2011年第5期1084-1091,共8页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(30800853);国家"十二五"科技支撑计划项目(2011BAD13B03);海洋公益性行业科研专项(200905020)

摘　　要：以仿刺参"化皮"病病原菌—灿烂弧菌(Vibrio splendidus)AP622为试验菌株,研究了培养材料、培养时间、培养基、葡萄糖浓度对菌株生物被膜形成能力的影响,证实鞭毛和菌毛介导的运动性,同时比较了浮游细菌与形成生物被膜的细菌对抗生素的敏感性,并研究了菌株的疏水性。结果表明,菌株AP622为高疏水性和高生物被膜形成菌株,在聚氯乙烯为培养材料、含葡萄糖浓度为0.5%、LB培养基中形成的生物被膜最多,生物被膜形成周期为24 h。菌株AP622明显表现出鞭毛介导的群集性和Ⅳ型菌毛介导的颤搐等运动力。生物被膜中的菌株AP622对抗生素的抵抗力明显强于浮游细菌,其最小抑菌浓度(MIC)是浮游细菌的32倍。综上所述,菌株AP622具有很强的疏水性和高生物被膜形成能力,判断其具有很强的黏附力,同时具有明显的抗药性。Vibrio splendidus infects a range of hosts,including fish,shellfish,and echinoderms.Infection with this pathogen has led to significant economic loss in several cultured species,including the sea cucumber（Aposti-chopus japonicus）.Thus,there is an urgent need to understand the pathogenesis of V.splendidus.The virulence of a pathogen is partly a function of its adhesion properties.Adhesion depends on cell surface hydrophobicity and biofilm formation.The hydrophobic cell surface provides an advantage to the bacteria in vivo by increasing resis-tance to phagocytosis or by favoring colonization of mucosal or connective tissue in wounds and endocarditis in bacteria.We evaluated the hydrophobicity and biofilm formation in V.splendidus AP622,isolated from diseased A.japonicus.We also evaluated the effect of culture constituents,culture time,growth medium,and the concentration of glucose on biofilm formation.We compared the sensitivity of planktonic cells and resuspended V.splendidus AP622 biofilm cells to gentamycin sulphate.Last,we quantified the motility of the flagellum and measured pill and cell surface hydrophobicity using tests for salt-aggregation（SAT） and microbial adherence to hydrocarbons（MATH）.V.splendidus AP622 possesses a high ability to form a biofilm.The biofilm formation cycle was 24 h in length and the optimal formation conditions consisted of polyvinyl chloride（PVC） and LB medium with 0.5% glucose.Swarming and twitching motilities played an important role in biofilm formation.The resistance of re-suspended biofilm bacteria to antibiotics was significantly higher than in planktonic bacteria（P〈0.05）.Furthermore,the minimum inhibitory concentration（MIC） was 32 times higher than in planktonic bacteria.V.splendidus AP622,exhibiting strong hydrophobicity,could agglutinate at a concentration of 0.6 mol/L ammonium persulfate,and the adhesion partition to liquid hydrocarbons was 50%.In conclusion,the hydrophobicity and biofilm formation properties suggest that V.splendidus AP622 has str

关 键 词：仿刺参 灿烂弧菌 生物被膜 运动性 疏水性 

分 类 号：S94[农业科学—水产养殖]