金属离子对甘油脱氢酶的化学修饰  

Chemical Modification of the Glycerol Dehydrogenase by Divalent Metal Ions

作　　者：郭英霞[1,2] 王世珍[1] 王兆守[1] 陈荣[2] 方柏山[1] 

机构地区：[1]厦门大学化学化工学院 [2]厦门大学海洋与环境学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2011年第5期883-889,共7页Journal of Xiamen University：Natural Science

基　　金：国家高技术研究发展计划(863)项目(2006AA020103);福建省高校产学合作科技重大项目(2010H6023)

摘　　要：甘油脱氢酶(GDH)可以催化甘油生成二羟基丙酮.为了进一步探索GDH的活性结构中心,提高GDH的活性,用Mg2+、Mn2+、Ba2+、Ca2+、Zn2+、Cu2+、Sr2+、Ni2+、Cd2+和Co2+等10种金属离子对GDH进行活性中心金属离子置换修饰,筛选出具有修饰效果的离子为Mg2+、Mn2+和Ba2+,修饰后其最大酶活力分别是初始酶活力的3.4,2.0和4.7倍.通过均匀设计对3种金属离子的修饰条件进行了优化,优化后测定的酶活力分别是未修饰GDH酶活力值3.17 U/mL的14.9,11.3和12.4倍,极大地提高了GDH的酶活力.Glycerol dehydrogenase （GDH） can catalyze the interconversion of glycerol and dihydroxyacetone. In order to improve theactivity and explore the active central site of GDH, 10 kinds of metal ions such as Mg^2＋ , Mn^2＋ , Ba^2＋ , Ca^2＋ , Zn^2＋ , Cu^2＋ ,Sr^2＋ , Ni^2＋ , CA^2＋ and Co^2＋ were used to do chemical modification on GDH. The results approved that the activity of GDH was significantly increased by Mg^2＋ ,Mn^2＋ and Ba^2＋ while the special activity of GDH was 3.4,2.0 and 4.7 folds respectively of the controlrs after modification. Meanwhile,the activity effects of Mg^2＋ ,Ba^2＋ and Mn^2＋ on GDH showed that the GDH was successfully modificated by Mg^2＋ ,Ba^2＋ and Mn^2＋. The modificated condition was optimized with the uniform design. Compared to the original activity of GDH 3.17 U/mL,the activity of GDH modified by Mg^2＋ ,Ba^2＋ and Mn^2＋was improved separately as 14.9,11.3 and 12.4 folds after optimization.

关 键 词：甘油脱氢酶 化学修饰 金属离子 辅酶 

分 类 号：Q554.1[生物学—生物化学]