暹罗鳄胆汁提取物对人胆管癌MZ-ChA-1细胞的抑制作用  

Effect of the Extraction from Crocodylus siamensis Bile on Proliferation of Human Bile Duct Carcinoma MZ-ChA-1 Cells

作　　者：宋巍[1] 沈东炎[1] 康劲翮[1] 李珊珊[1] 熊佑熊 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]泰国是拉差龙虎园有限公司

出　　处：《厦门大学学报（自然科学版）》2011年第5期938-941,共4页Journal of Xiamen University：Natural Science

基　　金：国家基础科学人才培养基金项目(J1030626);厦门市科技计划项目(3502Z20103008)

摘　　要：以暹罗鳄(Crocodylus siamensis)胆汁为材料,应用细胞培养、细胞计数、Hoechst33258染色、流式细胞仪等技术研究暹罗鳄胆汁提取物对人胆管癌MZ-ChA-1细胞凋亡的诱导作用.实验结果显示,胆汁提取物可明显诱导人胆管癌MZ-ChA-1细胞的凋亡,经1.75 mg/mL暹罗鳄胆汁提取物诱导处理24 h后,人胆管癌MZ-ChA-1细胞的增殖活动受到显著的抑制,细胞生长抑制率达86.71%;光镜观察结果显示,暹罗鳄胆汁提取物处理细胞后,细胞体积缩小,细胞核固缩;细胞核经Hoechst33258染色出现浓染致密的固缩形态和颗粒状荧光;细胞周期检测出现G0/G1期细胞比例升高,S和G2/M期细胞比例下降.研究结果表明,暹罗鳄胆汁提取物对人胆管癌MZ-ChA-1细胞凋亡的诱导具有显著作用,从而为进一步研究细胞凋亡机制提供重要基础和研究依据.The apoptosis effects of extraction from Crocodylus siamensis bile （ECB） on Human bile duct carcinoma MZ-ChA-1 cellshave been studied using the methods of cell culture, cell count, Hoechst33258 staining,flow cytometry analysis. The results showed that proliferation of MZ-ChA-1 cells could be inhibited by 1.75 mg/mL ECB after 24 h,and the inhibitory rate is 86.71 % Light microscope showed that the morphology of the cells treated with bile extracts appeared shrinked, cell nucleus concentrated. Cell nucleus concentrated and appeared granular fluorescence by Hoechst33258 stainning. The results of flow eytometry analysis showed that the phase of G0/G1 is increased and the phase of G2/M is decreased. This study suggested that ECB had induced apoptosis of MZ-ChA1 cells effectively,and provides important foundation and research proofs to study more about the apoptosis mechanisms of MZ-ChA1 cells.

关 键 词：暹罗鳄胆汁 胆管癌 细胞凋亡 

分 类 号：Q247[生物学—细胞生物学] Q28