莱菔硫烷诱导人肝癌HepG-2细胞凋亡的JNK途径  

Study on JNK pathway in human hapetocelluar carcinoma HepG-2 cells apoptosis induced by sulforaphane

作　　者：邹翔[1,2] 曲中原[2] 白晶[3] 季宇彬[1,2,3] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076 [3]哈尔滨商业大学中药学博士后流动站,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2011年第4期532-535,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金资助项目(30300284);黑龙江省自然科学基金项目(D200802);黑龙江省骨干教师项目(1154G36)

摘　　要：探讨莱菔硫烷(sulforaphane,SFN)诱导人肝癌HepG-2细胞凋亡过程中,JNK途径的作用.采用荧光显微镜观察凋亡细胞形态;采用Western Blot法检测人肝癌HepG-2细胞内JNK和p-JNK蛋白的表达.结果表明,10、20、40μmol/L的SFN作用人肝癌HepG-2细胞48 h后,荧光显微镜下可见随着药物浓度的增大,凋亡细胞数量逐渐增多,并呈现典型的凋亡细胞形态;HepG-2细胞内p-JNK蛋白表达量逐渐升高,而对JNK的表达无明显影响.提示SFN可诱导HepG-2细胞的发生凋亡,激活JNK信号通路可能是其主要机制之一.To investigate the roles of JNK pathway in apoptosis induced by sulforaphane（SFN） in human hapetocelluar carcinoma HepG-2 cells.Fluorescence microscope was used to observe the morphological changes of HepG-2 cells after treated by different dosage of SFN.Western Blot assay was employed to detect the expression of JNK and p-JNK proteins.The results showed that after treated by SFN at the dosage of 10,20,40 μmol/L for 48 h,the apoptotic cell numbers increased rapidly when the dose rose and showed typical apoptotic morphology.The expression of p-JNK protein was evidently up-regulated,meanwhile,there was no remarkable change on the expression of JNK protein.A conclusion can be drawn that SFN can induce cell apoptosis of HepG-2 cells by activating JNK pathway in HepG-2 cells.

关 键 词：莱菔硫烷 人肝癌HEPG-2细胞 JNK p—JNK 

分 类 号：R285[医药卫生—中药学]