乙肝病毒核心蛋白对TRAIL诱导细胞凋亡的抑制作用研究  

The inhibitting effect of hepatitis Brirus coreprotein on the apoptosis of hepatocytes induced by TRAIL

作　　者：张鹏[1] 刘爱云[1] 伊纪美 

机构地区：[1]山东省桓台县人民医院,山东桓台256400

出　　处：《医学检验与临床》2011年第4期22-24,共3页Medical Laboratory Science and Clinics

摘　　要：目的探讨HBV核心蛋白（HBe）对TRAIL诱导肝细胞凋亡的影响。方法建立能表达HBc蛋白的细胞模型，用生长曲线和TUNEL的方法检测HBc的表达对TRAIL诱导凋亡的影响。结果CCK-8检测结果显示，相同浓度TRAIL作用下，BEL7402-HBc细胞较对照组细胞的生长抑制率明显降低，当作用浓度为10ng／mL时，差异最为显著；TUNEL流式细胞术结果显示，10ng／mL TRAIL处理BELT402-HBe细胞后，与对照组细胞相比凋亡率明显降低（P〈0．01）。结论HBc表达可降低TRAIL诱导肝细胞凋亡的敏感性，证实HBe具有抵抗TRAIL诱导凋亡的生物学活性。Objective To demonstrate the effect of HBe on TRAIL- induced hepatocyte apoptesis. Methods Construct hepatoma cell model and to detect the effects of HBc on TRAIL - induced apoptosis use cell growth curve and TUNEL methods. Results CCK - 8 ashy showed that, treated with the same concentration of TRAIL, BELT402- HBc cells had lower cytotoxicity rates than control cells. The difference was most dramatic when the concentration of TRAIL was 10ng/mL. Results of TUNEL displayed that, treated with 10ng/mL TRAIL, BEL7402- HBc cells had lower apoptisis rates than control cells（ P 〈 0.01 ）. Conclusion HBe decreases the sensitivity of hepatoma cells towards TRAIL and firstly reports the downregulation of TRAIL - induced apoptosis by HBc.

关 键 词：TRAIL HBC 凋亡 

分 类 号：R392.11[医药卫生—免疫学]