亚甲基蓝共振瑞利散射法测定阿莫西林的含量  

Resonance Rayleigh Scattering Spectrometric Determination of Amoxicillin by Its Reaction with Methylene Blue

作　　者：江虹[1] 舒亚林 张秀菊[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100 [2]重庆市涪陵计量质量检测中心,重庆408000

出　　处：《理化检验（化学分册）》2011年第9期1063-1065,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：重庆市教委科技基金资助项目(No:KJ071304);长江师范学院科研资助项目(No:2008xky39)

摘　　要：在pH8．93的三羟甲基氨基甲烷-盐酸介质中，一定量的阿莫西林与1．0×10-5mol·L-1亚甲基蓝溶液1．5mL在总体积10mL中反应生成离子缔合物，产生共振瑞利散射光谱，在其最大共振光散射峰369nm波长处测定共振瑞利散射的强度jRRs。阿莫西林浓度在2．0×10-7～1．0×10-6mol·L-1范围内，与样品及空白溶液IRRS值之差△IRRS呈线性关系，方法的检出限（3s／k）为7．8×10-8mol·L-1方法用于药物中阿莫西林的测定，测得结果与药典法测得结果相符。In a Tris HCl buffer medium of pH 8. 93, an appropriate amount of amoxicillin sample solution was reacted with 1.5 mL of 1.0×10-5mol·L-1 methylene blue solution in a total volume of 10 mL, to form an ion association complex, of which the intensity of resonance Rayleigh scattering （IRRS） was measured at the wavelength of 369 nm. Values of △IRRS （difference between values of IRRs of sample and blank） were found to keep linear relationship with values of concentration of amoxicillin in the range of 2. 0 × 10 -7 - 1. 0 ×10-6mol·L-1 , with detection limit （3s/k） of 7. 8×10-6mol·L-1. The proposed method was applied to the determination of amoxicillin in samples of capsules and injections, results obtained by this method were found to check quite well with the results found by the method of pharmacopeia.

关 键 词：共振瑞利散射光谱法 阿莫西林 亚甲基蓝 

分 类 号：O657.3[理学—分析化学]