红豆树的组织培养技术  

Ormosia hosiei Tissue Culture Technique

作　　者：范辉华[1] 李朝晖[1] 张蕊[2] 陈柳英[3] 

机构地区：[1]福建省林业科学研究院,福建福州350012 [2]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [3]福建省建瓯市林业技术推广中心,福建建瓯353100

出　　处：《福建林业科技》2011年第3期100-102,120,共4页Journal of Fujian Forestry Science and Technology

基　　金：福建省公益类科研专项(2009R10008-2);中央级公益性科研院所基本科研专项(RISF6809)

摘　　要：以红豆树(Ormosia hosiei et Wils)优树根蘖苗移栽促萌形成枝条为外植体进行带牙茎段组织培养试验研究,结果表明:最佳外植体为半木质化嫩枝顶端的带牙茎段;最适诱导培养基为MS+BA 0.5 mg.L-1+NAA 0.05 mg.L-1,平均诱导率达52.05%;增殖培养阶段最适不定芽诱导培养基为MS+BA 2.0mg.L-1+NAA 0.1 mg.L-1。Ormosia hosiei dominant tree root suckers were transplanted to form branches by promoting sprouting,which were taken as explants for tissue culture test of a stem with buds,the results showed that：the best explant was the semi-woody twig top of the stem segments with buds;optimum induction medium was MS＋BA0.5 mg·L-1＋NAA0.05 mg·L-1,the average induction rate was 52.05%;the optimal adventitious induction medium at proliferation culture stage was MS＋BA2.0 mg·L-1＋ NAA0.1 mg·L-1.

关 键 词：红豆树 带牙茎段 组织培养 

分 类 号：S791.49[农业科学—林木遗传育种] S723.132.6[农业科学—林学]