p,p’-DDE对大鼠睾丸支持细胞细胞外信号调节激酶转导通路影响的机制研究  

Effect of Signal Transduction Pathway Induced by p,p'-DDE in Rat Sertoli Cells

作　　者：王翀[1] 张宏伟[1] 杨克敌[2] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021 [2]华中科技大学同济医学院公共卫生学院

出　　处：《环境与健康杂志》2011年第9期762-764,共3页Journal of Environment and Health

基　　金：国家973计划项目(2002CB512908)

摘　　要：目的研究p,p’-DDE对离体培养大鼠睾丸支持细胞(Sertoli cell)内的细胞外信号调节激酶(ERK)丝裂原活化蛋白激酶(MAPK)信号转导通路影响机制。方法从大鼠睾丸组织中分离支持细胞进行离体原代培养,分别以0、10、30、50μmol/L的p,p’-DDE染毒,用MTT比色法、SABC法和Western blotting法来检测细胞活性以及p-ERK的表达情况。结果在MTT试验中,支持细胞活性随着p,p’-DDE剂量的增高而逐渐降低;在免疫组化试验中,细胞内p-ERK随p,p’-DDE剂量的增高而染色加深;同时,在western blotting实验中50μmol/L p,p’-DDE染毒组支持细胞内p-ERK在染毒后12、24 h较染毒前表达上升,差异有统计学意义(P<0.05)。结论 p,p’-DDE导致支持细胞细胞活性降低;同时激活细胞内ERK磷酸化,并且随着作用时间的增加,ERK的磷酸化表达于12、24 h时明显增加,这预示着ERK磷酸化可能参与信号通路的传导,可能会影响下游通路进而影响到基因的转录和表达。Objective To study the effect of intracellular ERK MAPK signal transduction pathway induced by p,p＇-DDE in rat Sertoli cells in vitro. Methods Sertoli cells were separated from testicular tissue of rats and cultured with p,p＇ -DDE of 0,10,30, 50μmol/L respectively. The cellular viability and the changes of intraeellular p-ERK were determined by immunohistoehemical SABC,MTY colorimetry and Western blotting method. Results Intracellular p-ERK increased with the dose of p,p＇-DDE. Cellular viability decreased with the dose of p,p＇-DDE. The expression of p-ERK in Sertoli cell at 12 and 24 h of treatment in 50μmol/L group significantly up-regulated compared with the control. Conclusion p,p＇-DDE can reduce the viability of Sertoli cells and enhance phosphorylation of ERK , which may take part in the activation of the downstream path and affect the transcription and expression of genes.

关 键 词：P P’-DDE 支持细胞 细胞内p-ERK 

分 类 号：R994.6[医药卫生—毒理学]