八氯代二苯并二噁英对C57BL/6j雄性小鼠心肌基因表达谱的影响  

Effects of 1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin on Cardiac Gene Expression Profile in C57BL/6j Male Mice

作　　者：王耀峰[1,2] 陶春燕 马全祥[1] 杨杰[1,2] 袁向山[1] 毛泽善[1] 杨志军[1,2] 文小军[1] 

机构地区：[1]新乡医学院基础医学院,河南新乡453003 [2]新乡医学院三全学院 [3]新乡市第二人民医院麻醉科

出　　处：《环境与健康杂志》2011年第9期779-782,F0003,共5页Journal of Environment and Health

基　　金：河南省高等学校青年骨干教师资助计划(2009GGJS-151);河南省教育厅自然科学研究计划(2009A310008);新乡医学院高学历人才科研启动基金

摘　　要：目的探讨八氯代二苯并二噁英(1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin,OCDD)对小鼠心脏基因表达谱改变的影响。方法将20只7～8周龄SPF级雄性C57BL/6j小鼠按体重随机分为4组,分别为对照(玉米油)组和低(1.25×10-6 g/ml)、中(1.25×10-5 g/ml)、高(1.25×10-4 g/ml)浓度OCDD染毒组,每组5只。采取灌胃方式进行染毒,染毒容量为8 ml/kg,每天1次,连续染毒30 d。应用基因芯片技术比较不同浓度OCDD染毒组和对照组雄性小鼠心脏基因表达差异。结果共检测了小鼠心脏基因41 253个。与对照组相比,低浓度OCDD染毒组和对照组差异基因总数5 490个,其中,上调2 257个,下调3 233个;中浓度OCDD染毒组和对照组差异基因数2 776个,其中,上调941个,下调1 835个;高浓度OCDD染毒组和对照组的差异基因数14 911个,上调10 476个,下调4 435个。各浓度OCDD染毒组和对照组共同的2倍以上差异基因为288个。结论亚慢性OCDD染毒对小鼠的心脏相关基因表达造成影响,提示心脏可能是OCDD毒性作用的靶器官。Objective To investigate the effects of 1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin （OCDD） on the cardiac gene expression profile in the heart of mouse. Methods Twenty male C57BL/6j mice aged 7 or 8-weeks, SPF class, were randomly divided into four groups, 5 mice in each, as normal control group（maize oil）,low concentration group（ 1.25×10^-6 g/ml, OCDD）, moderate concentration group （1.25 × 10^-5 g/ml, OCDD）and high concentration group （ 1.25 ×l0^-4 g/ml, OCDD）. Pretreatment groups were given solvents by 8 ml/kg each day in the way of garage for 30 days. The differences of gene expression profile between OCDD exposed groups and control group in C57BL/6j male mice were compared by gene chip technology. Results A total of 41 253 heart genes were detected. Compared with the control group,in low OCDD exposure group, the number of different genes was 5 490,with 2 257 up-regulated and 3 233 down-regulated; in moderate OCDD exposure group, it was 2 776 ,with 941 up-regulated and 1 835 down-regulated; In high OCDD exposure group, it was 14 911, with 10 476 up-regulated and 4 435 down-regulated. The number of the different genes of more than two times both in OCDD exposure and the control groulSs was 288. Conclusion The effect of sub-chronic exposure to OCDD on the cardiac gene expression in male C57BL/6j mice indicates that the heart is probably the target organ of OCDD.

关 键 词：基因芯片 心肌 八氯代二苯并二英 

分 类 号：R994.6[医药卫生—毒理学]