久效磷对大鼠皮肤成纤维细胞胶原Ⅰ和ⅢmRNA表达的影响  

Effects of Monocrotophos on Expressions of Collagens TypeⅠ and Type Ⅲ in Rat Skin Fibroblast Cells

作　　者：周亚莉[1] 闫建国[2] 王晶晶[2] 朱振东[2] 苗莹莹[2] 

机构地区：[1]新乡医学院微生物学教研室,河南新乡453003 [2]新乡医学院人体解剖学教研室

出　　处：《环境与健康杂志》2011年第9期785-787,F0003,共4页Journal of Environment and Health

基　　金：新乡医学院高学历人才科研启动项目

摘　　要：目的研究久效磷对体外培养的大鼠皮肤成纤维细胞生长及胶原Ⅰ和Ⅲ合成的影响。方法取4只出生3 d清洁级SD大鼠背部皮肤,以组织块法培养大鼠皮肤成纤维细胞,正常传代。取第4代成纤维细胞,按1.0×106个/瓶接种于25 cm2培养瓶中,当细胞至亚融合状态,分别加入0(对照)、0.01、0.1、1μg/L的久效磷溶液,培养24 h,镜下观察细胞生长状况,收集各组细胞。采用实时定量PCR(RT-PCR)检测大鼠皮肤成纤维细胞胶原Ⅰ和ⅢmRNA的表达;采用噻唑兰(MTT)比色法检测皮肤成纤维细胞的细胞活性。结果久效磷对体外培养的皮肤成纤维细胞有显著的抑制作用,且抑制率呈剂量依赖性;0.01、0.1、1μg/L久效磷染毒组大鼠皮肤成纤维细胞抑制率均高于对照组,差异有统计学意义(P<0.05或P<0.01)。且随着久效磷染毒剂量的升高,大鼠皮肤成纤维细胞胶原Ⅰ和胶原ⅢmRNA的表达水平呈下降趋势;与对照组相比,0.01、0.1、1μg/L久效磷染毒组大鼠皮肤成纤维细胞胶原Ⅰ和胶原ⅢmRNA的表达水平均下降,差异有统计学意义(P<0.05或P<0.01)。结论久效磷可抑制大鼠皮肤成纤维细胞的生长,降低大鼠皮肤成纤维细胞胶原Ⅰ和ⅢmRNA的表达。Objective To investigate the effects of different concentrations of monocrotophos on growth and collagen synthesis of rat skin fibroblast cells in culture. Methods Rat skin fibroblast cells of P4 in culture were treated with monocrotophos at the doses of 0,0.01,0.1 and 1 μg/L for 24 h after 48 h of culture. Cell proliferation was detected by MTT method, and the mRNA expression of collagens type I and type Ⅲ were detected by RT-PCR. Results With the increase of monocrotophos concentration in culture medium, the growth of skin fibroblast cells was significantly inhibited. Compared with the controls, the mRNA expression of collagens type I and type Ⅲ were significantly decreased. Conclusion Monocrotophos can significantly inhibit the growth of rat skin fibroblasts in culture,and decrease the expression of collagens type I and type m mRNA in rat skin fibroblasts cells.

关 键 词：久效磷 皮肤成纤维细胞 胶原I 胶原Ⅲ 大鼠 

分 类 号：R994.6[医药卫生—毒理学]