线粒体细胞色素c介导的caspase-9激活通路参与大骨节病关节软骨细胞凋亡的发生机制  

Mitochondrial cytochrome c mediated caspase-9 activation pathway plays an important role in chondrocytes apoptosis of Kashin-Beck disease

作　　者：刘江涛[1,2] 郭雄[1] 吴翠艳[1] 马玮娟[1] 韩晶[1] 武世勋[1,2] 张银刚[2] 

机构地区：[1]西安交通大学医学院公共卫生系教育部环境与疾病相关基因重点实验室,陕西西安710061 [2]西安交通大学医学院第一附属医院骨科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2011年第5期580-584,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30972556);高等学校博士点科研专项基金(No.20090201110049)~~

摘　　要：目的通过评价线粒体功能研究线粒体介导的caspase-9激活通路参与成人大骨节病关节软骨细胞凋亡的机制。方法关节软骨细胞培养,Hoechst 33258染色检测软骨细胞核形态,流式细胞仪检测软骨细胞凋亡率和细胞内活性氧含量,Western bloting检测细胞色素c的表达,荧光法检测caspase-9和caspase-3活性。结果大骨节病患者关节软骨细胞较正常软骨细胞凋亡百分率增加,线粒体细胞色素c释放,caspase-9和caspase-3活性增高,且活性氧含量增加。结论线粒体氧化应激参与了大骨节病软骨细胞的病理生理变化,线粒体功能障碍可能在大骨节病关节软骨细胞凋亡过程发挥了重要作用。Objective To evaluate the mitochondrial function to understand the mitochondria-mediated caspase-9 activation and apoptosis in adult Kashin-Beck disease（KBD） chondrocytes.Methods Apoptotic cell was evaluated by analyzing the apoptosis rate of KBD articular chondrocytes and the caspase-9 and 3 activities.Mitochondrial function was evaluated by analyzing the cytochrome c release from mitochondria to the cytosol and the intracellular reactive oxygen species（ROS） contents.Results The percentage of positive apoptotic chondrocytes from the KBD patient group exhibited a higher level than that of the healthy controls.Mitochondrial cytochrome c release and caspase-9 and 3 activation were also found.Conclusion These findings suggest the involvement of mitochondrial oxidative stress and celluar apoptosis in the pathophysiology of KBD.The dysfunction of mitochondria may play an important role in KBD articular chondrocytes apoptosis.

关 键 词：线粒体 细胞凋亡 软骨细胞 大骨节病 细胞色素C CASPASE-9 CASPASE-3 活性氧(ROS) 

分 类 号：R681[医药卫生—骨科学] R683[医药卫生—外科学]