高压氧对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-2表达及血脑屏障通透性的影响  

Effects of hyperbaric oxygen on the expression of caveolin-2 in brain tissues and the blood brain barrier after focal cerebral ischemia and reperfusion

作　　者：赵红[1] 张倩茹[1] 张海鹏[2] 陈学新[3] 

机构地区：[1]中国医科大学基础医学院机能实验中心,沈阳110001 [2]中国医科大学基础医学院病理生理教研室,沈阳110001 [3]中国医科大学附属第一临床医院高压氧科

出　　处：《中华物理医学与康复杂志》2011年第9期652-655,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：辽宁省教育厅高等学校科研基金资助项目（2005L456）

摘　　要：目的研究高压氧（HBO）对局灶性脑缺血再灌注（I／R）大鼠脑组织小窝蛋白-2（caveolin-2）、基质金属蛋白酶-9（MMP-9）表达及血脑屏障（BBB）通透性的影响。方法将雄性Wistar大鼠370只分成假手术组80只、I／R组130只、HBO组80只、HBO-4-I／R组80只。采用线栓法建立大鼠局灶性脑缺血再灌注模型。HBO组和HBO＋I／R组于再灌注0，2，9，21，45，69h进入HBO舱，经0．25MPaHBO治疗5次。采用比色法、免疫组化法及Westernblot法检测BBB的通透性、caveolin-2及MMP-9的表达。结果第4，12，24，48，72小时脑组织伊文思兰（EB）的含量与0h组相比明显增加，再灌注后4h脑组织EB的含量最高。HBO＋I／R组脑组织EB的含量明显低于I／R组。HBO组与假手术组相比脑组织EB的含量无明显变化。脑缺血再灌注第24，48，72小时caveolin-2、MMP-9表达明显高于0h组。HBO十I／R组与I／R组相比脑组织caveolin-2、MMP-9表达显著减低。HBO组脑组织caveolin-2、MMP-9表达与假手术组相比无明显变化。结论HBO降低脑缺血再灌注时增高的脑微血管内皮细胞caveolin-2和MMP-9的表达，从而降低BBB通透性。Objective To investigate the effects of hyperbaric oxygen （HBO） on the expression of caveolin- 2 and matrix-metalloproteinase-9 （MMP-9） in brain tissues and the blood brain barrier （BBB） after cerebral ischemi- a and reperfusion （I/R） in rats. Methods Three hundred and seventy male Wistar rats were randomly assigned into sham, ischemia-reperfusion, HBO, and I/R ＋ HBO groups. After creating cerebral I/R models, oxygen at 0.25 MPa was administered 5 times, and 2% Evans blue （EB） was injected into the tail veins 1 h before the rats were sacrificed. The permeability of the BBB, the expression of caveolin-2 and MMP-9, and EB content were determined by Western blotting, immunohistochemistry, and spectrophotometery, respectively. Results In the I/R group, the EB content increased steadily to a peak at 4 hours. EB content in the IR ＋ HBO group was significantly lower than in the I/R group. Caveolin-2 and MMP-9 were significantly augmented by I/R injury at the 24th, 48th and 72rid hours. Compared to the I/R group, HBO intervention decreased their expression levels. Conclusion HBO intervention can reverse the increase of caveolin-2 and MMP-9 caused by 1/R injury, which suggests a mechanism for protective effects of HBO on the permeability of the BBB in I/R injury.

关 键 词：高压氧 脑缺血再灌注 血脑屏障 小窝蛋白-2 基质金属蛋白酶-9 大鼠 

分 类 号：R285.5[医药卫生—中药学]