高能灌注液对断肢再植术后肢体保护作用的实验研究  

Prophylactic effects of high energy solution on extremity after limb replantation

作　　者：孙海波[1] 魏俊强[1] 陈伟[1] 张英泽[1] 郑占乐[1] 潘进社[1] 

机构地区：[1]河北医科大学第三医院创伤急救中心,石家庄050051

出　　处：《中国矫形外科杂志》2011年第18期1542-1546,共5页Orthopedic Journal of China

基　　金：河北省自然科学基金资助项目<延时高位断肢再植早期处理措施实验研究>;(编号:C2008000926)

摘　　要：[目的]探讨高能灌注液对断肢再植术后肢体保护作用。[方法]选用健康SD兔18只,根据干预方法的不同将18只SD兔随机分为3组,每组6只,为空白对照组、缺血再灌注组(IR组)、高能灌注液保护组(高能组)。建立左后离断肢体模型,高能组于缺血时、再灌注时给予高能灌注液灌注,于2、4、8、12 h于胫骨前肌取材,测定骨骼肌超氧化物歧化酶(SOD)、丙二醛(MDA)、髓过氧化物酶(MPO)、Na+-K+-ATP酶、Ca2+-ATP酶,湿重/干重比值,光镜下观察大体结构变化。[结果]各项指标经过统计学处理后,发现三组间有着显著性差异(P<0.05),高能组明显优于缺血再灌注组,并且随着时间的延长差异更加明显。[结论]高能灌注液能够有效地预防骨骼肌的缺血再灌注损伤,从不同环节进行多种药物联合应用是对断肢再植术后肢体保护的有效方法。[ Objective] To investigate the prophylactic effects of high energy solution on extremity after limb replantation. [Method] Eighteen healthy SD rabbits were chosen to set up replantation models in the left hindlimbs. According to different interventions,the rabbits were randomly divided into 3 groups：control group,ischemia-reperfusion injury group and high energy solution group. In high energy solution group, before the blood circulation was resumed, the rabbits were given high energy solution, about 0.1 gram of tibialis anterior muscles tissues of rabbits were preversed, and superoxide dismutase, malondialdehyde, myeloperoxidase, Na＋ - K＋ - ATP asc,Ca2＋ - ATP ase, W/D were analyzed,and morphology of tibialis anterior muscles were observed under microscope. [ Result] All indexes showed that every group had statistical difference（ P 〈 0.05 ）. The indexes in high energy solution group were significantly better than those of ischemia repeffusion injury group. [Conclusion] High energy peffusion solution cau improve the ischemia-reperfusion to the extremity after limb replantation. Multiple use of medMne is more effective to prevent ischemia-reperfusion injury.

关 键 词：高能灌注液 缺血再灌注损伤 断肢(指)再植 兔子 

分 类 号：R658.1[医药卫生—外科学]