肺岩宁方组分对小鼠肿瘤血管生成与MMP-2、MMP-9关系的研究  

The Relationships between Angiogenesis and Matrix Metalloproteinase-2,-9 in Mice Tumor Intervened by Feiyanning Recipe's Components

作　　者：王中奇[1] 邓海滨[1] 吴继[1] 张丽曼[1] 徐振晔[1] 

机构地区：[1]上海中医药大学附属龙华医院肿瘤科,上海200032

出　　处：《中国中西医结合杂志》2011年第9期1229-1233,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：上海市教育委员会科研创新项目(No.08YZ61);上海市浦东新区中医领军型人才项目(No.PWZl2009-04)

摘　　要：目的观察中药肺岩宁方组分对C57BL/6小鼠Lewis肿瘤微血管密度(microvessel density,MVD)与基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)、MMP-9mRNA及蛋白表达的影响。方法建立C57BL/6小鼠Lewis肺癌模型,随机分为模型组、肺岩宁组、益气组、补肾组和抗癌组。灌胃21天,第22天处死,取肿瘤组织,链霉菌抗生物素蛋白-过氧化物酶连接法(SP法)检测C57BL/6小鼠Lewis肿瘤MVD,逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)法和SP法检测MMP-2、MMP-9mRNA及蛋白表达水平。结果肺岩宁组、补肾组、抗癌组小鼠肿瘤MVD均较模型组和益气组显著降低(P<0·01,P<0·05),而益气组与模型组比较,差异无统计学意义(P>0·05)。肺岩宁及其拆方的各组MMP-2mRNA和蛋白(除益气组外)表达显著低于模型组(P<0·05,P<0·01),补肾组、抗癌组肿瘤MMP-2mRNA表达也显著低于益气组(P<0·05);肺岩宁组、补肾组、抗癌组的小鼠肿瘤MMP-9mRNA和蛋白表达均显著低于模型组和益气组(P<0·05,P<0·01)。结论补肾、抗癌中药抑制肿瘤血管生成的靶点可能与抑制MMP-2、MMP-9表达、保护肿瘤微环境有关。Objective To observe the effects of Feiyanning Recipe＇s（FR） components on the microvessel density（MVD）,the mRNA and protein expressions of matrix metalloproteinase-2（MMP-2） and MMP-9 in Lewis tumors loaded in C57BL/6 mice.Methods C57BL/6 Lewis lung cancer mouse model was established.Mice were randomly divided into five groups,i.e.,the model group,the FR group,the qi benefiting group,the Shen-tonifying group,and the anti-cancer group.The mice were killed on the 22nd day after 21-day gastrogavage.Tumors were extracted.The MVD of Lewis tumor was detected by immunohistochemical SP method.The mRNA and protein expressions of MMP-2 and MMP-9 were measured using reverse transcription-polymerase chain reaction（RT-PCR） and SP method.Results Compared with the model group and the qi benefiting group,the MVD was significantly reduced in the FR group,the Shen-tonifying group,and the anti-cancer group（P0.01,P0.05）.But there was no significant difference between the qi benefiting group and the model group（P0.05）.The mRNA and protein expressions of MMP-2 in the FR group,the Shen-tonifying group,and the anti-cancer group were also significantly less than those in the model group（P0.05,P0.01）.At the same time the expression of MMP-2 mRNA in the Shen-tonifying group and the anti-cancer group was also less than that in the qi benefiting group（P0.05）.The mRNA and protein expressions of MMP-9 in the FR group,the Shen-tonifying group,and the anti-cancer group were significantly lower than those in the model group and the qi benefiting group（P0.05,P0.01）.Conclusion The target for Shen-tonifying and anti-cancer Chinese herbs to inhibit tumor angiogenesis might be correlated with restraining expressions of MMP-2 and MMP-9,and protecting tumor microenvironment.

关 键 词：肺岩宁方 LEWIS肺癌 血管生成 微血管密度 基质金属蛋白酶-2 基质金属蛋白酶-9 

分 类 号：R285[医药卫生—中药学]