含苯聚羟基脂肪酸酯的生物合成及其分子结构表征  

Biosynthesis and molecular structure characterization of polyhydroxyalkanoates containing benzoyl group

作　　者：黄锦标[1,2] 尚龙安[1] 

机构地区：[1]浙江大学宁波理工学院,浙江宁波315100 [2]浙江大学化学工程与生物工程学系,浙江杭州310027

出　　处：《化工进展》2011年第10期2282-2286,共5页Chemical Industry and Engineering Progress

基　　金：宁波市自然科学基金项目(2006A61003)

摘　　要：聚羟基脂肪酸酯(PHA)具有可生物降解性和生物相容性,是潜在的医学材料。对聚羟基脂肪酸酯进行改性,可提高其应用范围.采用以苯戊酸和蔗糖为混合碳源培养基培养Pseudomonas putida KT2442,对其细胞内合成的聚羟基脂肪酸酯进行生物改性,并利用核磁共振、红外色谱和气相色谱分析方法表征了改性的PHA分子结构。结果表明合成的聚羟基脂肪酸酯为含有3-羟基苯戊酸单体的中长链聚羟基脂肪酸酯(PHPhA),该单体在PHA链中含量为1.04%,为进一步的研究打下基础。Polyhydroxyalkanoates（PHA）with the biodegradability and biocompatibility has become one of the potential medical materials.Its application fields can be enlarged by modifying the PHA＇s properties.For this purpose,the PHA was synthesized by the culture of Pseudomonas putida KT2442 with phenylvaleric acid and sucrose as mixed carbon source.The molecular structure of this biomodified PHA was characterized by NMR,IR and GC.The analysis result indicates that the biomodificated PHA is medium-length PHA which contains 3-hydroxyphenylvalerate monomer,and this monomer accounts for 1.04% of the PHA chain.This will be useful reference for further PHA study.

关 键 词：聚羟基脂肪酸酯 生物改性 恶臭假单胞菌 混合碳源 

分 类 号：TQ323.4[化学工程—合成树脂塑料工业]